摘要
建立了一种快速、准确、灵敏的固相萃取-离子对反相高效液相色谱测定保健品中叶酸的方法。用Chromabond-SB萃取小柱富集保健品中的叶酸,以四丁基溴化铵为离子对试剂,优化了色谱条件。采用Lu-NaC18色谱柱(250mm×4.6mm,5μm),SPD-6AV紫外可见吸收检测器,流动相为0.05mol/LKH2PO4-0.01mol/L四丁基溴化铵-乙腈(88:2:10,V/V),用磷酸调节pH5.5,流速1.0ml/min,检测波长284nm。结果表明:叶酸在0.06～2.40μg/ml浓度范围内,峰面积和浓度呈良好的线性关系,相关系数为0.9996,最小检出限为0.008μg/ml,加标回收率为95.14%～102.87%。
A simple, rapid, accurate and sensitive method of solid-phase extraction and ion-pair RP-HPLC was developed for the determination of folic acid （FA） in health food products. Solid-phase extraction （SPE） was performed to enrich FA extracted using a Chromabond-SB cartridge with strong anion exchange material. The addition of tetra-n-butylammoniurn, as an ion-pairing reagent, from samples was found to significantly increase the retention time of FA, thus leading to a well shaped and clearly separated single peak. The analysis was performed on a Lu-Na C18 column （5μ m, 250 mm × 4.6 mm） using a mobile phase composed of 0.05 mol/L KH2PO4 solution, 0.01mol/L tela-a-n-butylammonium and acetonitrile （88：2：10, V/V, pH 5.5） at a flow rate of 1 ml/min, with the UV detection set at 284 nm. In the range of 0.06 - 2.40μ g/ml, a good linear relationship between peak area and FA concentration was found, and the correlation coefficient and the LOD were 0.9996 and 0.008 μg/ml, respectively. The spike recoveries for FA in three commercial health food products ranged from 95.14% to 102.87%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第14期171-174,共4页
Food Science
基金
国家自然科学基金项目(20675024)
第三十七批中国博士后科学基金项目(2005037629)
河北省教育厅科学计划项目(2006407)
关键词
固相萃取
HPLC
保健品
叶酸
solid-phase extraction
HPLC
health food products
folic acid
