摘要
１９９６年４～５月山东省莱州市大华水产实业公司育苗场海湾扇贝亲贝发生了一起严重的流行病。这种病的主要症状是肠道及肾肿胀、生殖腺及外套膜萎缩、壳内面变黑。自发病亲贝肾组织中分离到细菌作纯培养，经肌肉注射和浸浴法人工感染健康亲贝，得到与发病池中患病亲贝相同的症状，并从人工感染病贝的肾组织中重新分离到同种细菌，证明所分离菌是发病亲贝的病原菌。经６０项形态及生理生化特性鉴定为飘浮弧菌（Ｖｉｂｒｉｏｎａｔｒｉｅｇｅｎ）。应用药敏纸片法研究了４３种化学疗剂对所分离的扇贝病原菌生长的抑制作用，其中新霉素Ｂ（１００μｇ／片）、氯霉素（３０μｇ／片）、磺胺药＋ＴＭＰ（２０μｇ／片）、美洛西林（３０μｇ／片）及多粘菌素Ｂ（３００ｉｕ／片）的抑菌力最强。
An epidemic disease of adult scallops occurred in Dahua Hatchery, Laizhou, Shandong Province during April to May, 1996. The symptoms of this disease were inflammation of digestive tract and kidney, atrophy of gonad and mantle, and presence of black pigments on inner side of the crust. One strain of bacteria was isolated from the kidney of a moribund scallop. Challenge experiments showed that this isolate was the pathogen of the disease. The isolate was tested for 60 unit characters and classified as Vibrio natriegen based on the criteria given by Alsina & Blach. The isolated bacteria were Gram-negative short rods with a single polar flagellum, and were sensitive to O/129 150 μg/ml. Oxidase and Catalase were positive. Glucose was fermented without the production of gas. Growth occurred in 0% NaCl and 10% NaCl(w/v) media. MR test and Indole test were negative. Argine decarboxylase, lysine decarboxylase, ornithine decarboxylase and phenylalaninase were not produced. Amylase, Lipase, Glatinase, Lecithinase and β-galactosidase were produced. Chitinase, Caseinase and Urease were not produced. Arabinose, Inositol, D-Raffinose, Sucrose, Mannitol, Lactose, Salicin, Sorbitol, α-Glucosamine and Amygdaline were fermented. V. natriegen was first reported as the causative agent of scallop disease.
基金
联合国教科文组织(UNESCO)资助
关键词
海湾
扇贝
病原菌
飘浮弧菌
化学疗剂
Argopecten irradins Lamarck
Pathogen
Vibrio natriegen
ChemotheratpeutantAcknowledgements The authors thank the UNESCO for support of this research (Contract number:SC/RP 206.950.5)
