摘要
目的探讨大鼠心肌梗死(MI)后细胞因子在心室重塑的表达及其与重塑的关系。方法结扎冠状动脉左前降支建立心肌梗死(MI)模型并设立假手术对照组在术后3d、1、4和24周检测血流动力学和组织形态学及胶原分子在心肌的分布表达变化、胚胎基因(β肌球蛋白重链),Ⅰ、Ⅲ型胶原和炎症因子的mRNA水平。结果与假手术组相比,MI组血流动力学改变明显。心肌梗死后第3天胚胎基因β-MHC、胶原、TGF-β1、TNF-α都上升,一直持续到第4周。梗死区Ⅰ,Ⅲ型胶原在第4周仍然比非梗死区为高,即使在第24周非梗死区的胶原水平仍然高于假手术组。TGF-β1、TNF-α在第1周达到高峰后逐渐下降,到第24周时非梗死区与假手术组相比差异有显著性(P<0.01)。相关性分析提示梗死区TGF-β1、TNF-α与Ⅰ,Ⅲ型胶原、β-MHC相关;非梗死区TGF-β1、TNF-α与Ⅰ,Ⅲ型胶原存在相关。结论细胞因子参与心室重塑病理生理过程并且可能是心室重塑的病因之一。
[Objective] To analyze the expression of cytokine in the ventricular remodeling of rat after myocardial infarction and reveal the relationship between them. [Methods] MI model was achieved by ligating the left anterior descending coronary artery and sham operated weeks. Expression of collagen type I and m was predominant in the infracted zone than that of in non-infarcted zone while it was higher than sham groups in the non-infarcted zone at 24 weeks. Compared to the sham operated groups, the levels of TGF-β1 and TNF-α reached their peak at 4 weeks, and then decreased gradually. The levels of cytokine in the 24 weeks were significantly higher than the sham groups in the non-infracted zone （P 〈0.01）. Correlation analysis of the mRNA expression indicated that fetal gene β-MHC, α- MHC, collagen subtype I , m, were correlated positively significantly to pro-inflammatory factor （TGF-β1）as well as pro-fibrosis （TNF-α） cytokine wherever in infracted and non-infracted zone （P 〈0.01）. [Conclusions] Cytokines participated in the myocardial remodeling of myocardial infarction at least, which was the one of the possible pathogenesis.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2009年第12期1821-1824,1828,共5页
China Journal of Modern Medicine
关键词
心肌梗死
心室重塑
细胞因子
myocardial infarction
myocardial remodeling
cytokine
