摘要
将海藻酸钠-壳聚糖固定化磷脂酶用于水化大豆油脱胶,通过单因素实验和旋转正交实验,确定最佳脱胶条件为:固定化酶添加量0.012%,起始pH 5.8,反应温度58℃,反应时间4 h。在最优条件下进行脱胶实验,测定固定化酶法脱胶油中的磷含量为3.85 mg/kg,脱胶效果较好。同时,固定化磷脂酶热稳定性提高,对反应环境的耐受力明显增强,使脱胶反应条件更易控制,是一种非常有效的脱胶方法。
The sodium alginate -chitosan immobilized phospholipase （PLA） was used for degumming of soybean oil. Through single factor test and orthogonal test, the optimal reaction conditions were determined as follows ： immobilized enzyme dosage 0. 012%, initial pH 5.8, reaction temperature 58℃, reaction time 4 h. Under the optimal conditions, the phosphorus content reduced to 3.85 mg/kg in degummed soybean oil. In addition, the thermal stabilities and the tolerance to the reaction conditions of immobilized PLA were enhanced, and the degumming conditions in practical production were easy to control.
出处
《中国油脂》
CAS
CSCD
北大核心
2009年第7期1-4,共4页
China Oils and Fats
基金
黑龙江省科技攻关计划项目"大豆综合利用关键技术研究与开发"(GA06B402-7)
关键词
大豆油
固定化磷脂酶
非水化磷脂
脱胶
soybean oil
immobilized PLA
nonhydratable phospholipids
degumming
