摘要
目的探讨pN0期食管鳞癌的血液微转移临床意义及其对预后的影响。方法提取血液标本总RNA，将mRNA反转录成cDNA，然后用实时荧光定量RT—PCR技术检测血液标本MMP-7 mRNA和hTERT mRNA表达，计算样本的△△Ct值，对40例N0期食管癌患者分别进行术后3、6、12个月跟踪随访复查。结果血液微转移与年龄、性别、癌肿长度等因素无相关关系，但与组织分化等级和pTNM分期间存在相关关系。随访结果显示单纯观察肿瘤不同侵犯深度与肿瘤术后转移（复发）率无相关性；随访术后6个月及12个月，血液微转移与否与肿瘤转移（复发）率有相关性（P〈0．05），其中血液微转移阳性患者术后12个月肿瘤转移（复发）概率是阴性患者的6．44倍（OR=6．440，95％CI为1．547～26．822）。结论食管鳞癌患者血液微转移检测对早期诊断及预后判断具有重要意义。
Objective To explore the clinical significance and the impact on prognosis of blood mierometastasis in the patients with pN0 esophageal squamous cell carcinoma. Methods Total RNA was extracted with TRIzol and mRNA was transcribed reversely into cDNA. RT-PCR was used to detect MMP-7 mRNA and hTERT mRNA in blood. △△Ct sample values were calculated with post-operative follow-up of 3 month, 6 month, 12 month. Results Statistical results suggested that blood micrometastasis was related to differentiation grade and pTNM staging （P =0.000, P =0.000 respectively）, but not to age, sex, length of tumour （P 〉0.05）. Follow-up results suggested that the degree of invasion and tumor metastasis （recurrence） was no correlation; follow-up to 6 month and 12 month, tumor metastasis （recurrence） was associated with blood micrometastasis, and follow-up to 12 month, compared with the tumor metastasis （recurrence） probability of blood micrometastasis-positive patients and negative patients, the former was as 6.44 times as the latter. （OR = 6.440, 95 % CI 1.547-26,822）. Conclusion Blood micrometastasis testing is of great significance to early diagnosis and prognosis judgment in pN0 esophageal squamous cell carcinoma patients.
出处
《肿瘤研究与临床》
CAS
2009年第7期450-452,共3页
Cancer Research and Clinic
