摘要
用HPLC法采用C18柱分离,流动相为甲醇:3%磷酸溶液=8∶2(体积分数),检测波长220nm,流速1.0mL/min,柱温为30℃测定合成氧化苦参碱的含量。结果表明:氧化苦参碱在1～10μg范围内,进样量与峰面积呈良好的线性关系(r=0.9998),氧化苦参碱的平均回收率为99.33%(n=6),RSD为0.296%。建立的HPLC方法灵敏、准确、重现性好,操作简便,可有效测定氧化苦参碱的含量。
To establish a method for determination of oxymatrine with HPLC, a C18 column and the mobile phase of MeOH-3%H3 PO4 （8 ： 2,v ： v） were used. The detection wavelength and temperature were set at 220 nm and 30℃, and the flow rate was 1.0 mL/min. The calibration curve of oxymatrine content was linear within the range of 1-10 μg （r=0. 9998）, the average recovery of oxymatrine was 99.33%, and RSD was 0. 296%. The HPLC method is accurate and sensitive for determining oxymatrine.
出处
《化学世界》
CAS
CSCD
北大核心
2009年第7期410-411,共2页
Chemical World
