摘要
重组蛋白在大肠杆菌中表达多为无活性的包涵体形式,须经洗涤、溶解、复性后才能得到生物活性蛋白。综述了近年来包涵体蛋白分离纯化和复性技术研究进展,重点讨论了色谱法复性技术的应用,包括尺寸排阻色谱、亲和色谱、离子交换色谱、疏水相互作用色谱、固定化脂质体色谱、扩张床吸附色谱的进展情况。
Expression of recombinant proteins in Escherichia coli often results inclusion bodies, an aggregation form of inactive proteins. Functional proteins can only be gained through a series of steps including washing, solubilization and refolding. The inclusion body purification strategy and chromatographic refolding techniques including size exclusion chromatography, affinity chromatography, ion-exchange chromatography, hydrophobic interaction chromatography, immobilized liposome chromatography, and expanded bed adsorption chromatography were summarized in this review.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2009年第7期102-107,共6页
China Biotechnology
基金
国家自然科学基金资助项目(30500347&30471212)
关键词
包涵体
纯化
色谱
复性
Inclusion body Purification Chromatography Renaturation
