摘要
目的:探讨细胞因子诱导杀伤细胞(cytokine-induced killer cell,CIK)对裸鼠胃癌移植瘤的靶向抑制作用。方法:将人胃癌细胞SGC-7901注射到裸鼠腹股沟皮下建立胃癌移植瘤裸鼠模型,荷瘤裸鼠随机分为CIK细胞组与成纤维细胞组,分别注射荧光染料SP-DiI标记的CIK细胞与成纤维细胞(HFL-I)于裸鼠种植瘤对侧腹股沟皮下,观察其在荷胃癌裸鼠体内各种组织中的分布情况;同时观察CIK治疗后肿瘤的体积大小并计算抑瘤率,病理观察肿瘤的坏死面积。结果:SP-DiI标记的CIK细胞注射后10d主要浓集在荷瘤裸鼠的肿瘤组织,注射局部、肝脏、脾脏和肺脏组织中无CIK细胞或分布极少(P<0.01);标记的成纤维细胞没有出现在肿瘤组织、肝脏、脾脏和肺脏组织,主要集中于注射局部。CIK细胞治疗后裸鼠的移植瘤体积显著小于对照组(P<0.05),其抑瘤率为29.82%;移植瘤组织坏死面积评分显著高于对照组(P<0.01)。结论:CIK细胞对裸鼠胃癌移植瘤有良好的靶向性和杀伤性。
Objective ： To investigate the inhibitory effect of cytokine-induced killer cells （CIK） against implanted gastric cancer cells. Methods： Gastric cancer 5GC-7901 cells were subcutaneously injected into the inguina of nude mice to establish gastric cancer model. The tumor bearing mice were randomly divided into CIK group and fibroblasts group, in which mice were subcutaneously injected with fluorescence dye SP-DiI labeled CIK and fibroblasts HFL-I cells, respectively. Distribution of CIK and HFL-I cells in different tissues of gastric cancer bearing mice were observed. Meanwhile, tumor volume was measured after different treatments and tumor inhibitory rate was calculated. Tumor necrosis areas in different groups were observed. Results： SP-DiI labeled CIK was mainly located in the gastric cancer tissues 10 d after injec- tion, and was hardly detected at the injection sites, liver, spleen and lung tissues （P 〈 0.01 ） ; SP-DiI labeled fibroblasts were not found in tumors, liver, spleen and lung tissues, and were mainly located in the injection sites. Volume of implanted tumor in CIK treated mice was significantly smaller than that in the control group （P 〈 0.05 ） , and tumor inhibitory rate of CIK group was 29.82%. The necrosis area score of implanted tumors was significantly higher in CIK group compared with that in the control group （P 〈 0.01 ）. Conclusion： CIK exhibits satisfactory ability to specifically kill implanted gastric cancer.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2009年第3期267-271,共5页
Chinese Journal of Cancer Biotherapy
基金
山东省自然科学基金资助项目(No.Y2006C22)~~
关键词
细胞因子诱导杀伤细胞
胃癌
移植瘤
靶向
杀伤
cytokine-induced killer cell
gastric neoplasms
implanted tumor
target
cytotoxicity
