摘要
目的 研究IL-24对细胞因子诱导的杀伤细胞（CIK）体内杀伤肿瘤细胞活性的影响.方法 （1）从健康人外周血中提取单个核细胞,第一天加入IFN-γ（1 000 IU/mL）,第二天加入IL-1（100 IU/mL）、CD3mAb（50 ng/mL）、IL-2（300 IU/mL）诱导CIK细胞,另一组与IL-1、CD3mAb、IL-2同时加入IL-24（1 000 IU/mL）;（2）建立HepG2肿瘤细胞株荷瘤小鼠皮下移植瘤模型,分为五组,每三天分别测量肿瘤大小.结果 IL-24诱导CIK细胞治疗组荷瘤小鼠实体瘤体积小于其它组（P＜0.05）.结论 在CIK细胞诱导过程中加入IL-24能明显增强其体内的抗肿瘤活性,对临床上把CIK细胞用于生物治疗有一定的指导意义.
Objective To study the effects of IL- 24 on eytotoxic activity of cytokineinduced killer cells in vivo. Methods （ 1 ） Mononuelear cells were extract from health aduh peripheral blood, one group added IFN -γ（ 1 000 IU/mL）at the first day and IL - 1 （ 100 IU/mL） ,CD3mAb（ 50 ng/mL） ,IL -2（ 300 IU/mL）at the second day. The other group added IL- 24 （ 1 000 IU/mL）at the same time with IL - 1, CD3mAb,IL -2;（ 2 ） Tumorbearing mice were divided into five groups,and tumor volumes were measured every three days. Results The solid tumor volumes of IL - 24 induced group are smaller than other groups （ P 〈 0.05 ）. Conclusion Added Ⅱ, - 24 during induced procedure can obviously enhance the cytotoxie activity of CIK cells in vivo.
出处
《实用肿瘤学杂志》
CAS
2011年第6期525-527,共3页
Practical Oncology Journal
基金
黑龙江省重点攻关课题基金资助（GOOC190401）
