摘要
目的探索p16基因甲基化状态与结直肠癌(CRC)早期诊断的可行性。方法从61例CRC患者、21例腺瘤患者和20例正常对照者粪便中提取DNA,采用巢式甲基化特异性PCR(nMSP)技术分析其p16基因甲基化改变状态。结果CRC、腺瘤、正常对照者粪便标本p16基因甲基化检出率分别为77.0%(47/61)、59.3%(16/27)、5.0%(1/20)。检测p16基因甲基化诊断CRC的敏感性和特异性分别为71.6%和95.0%。p16基因甲基化的阳性状态与CRC患者年龄、性别、肿瘤发生部位无明显关系,p16基因甲基化的阳性状态与肿瘤的Dukes分期、组织分化程度和淋巴结转移有关。结论p16基因甲基化改变发生在CRC形成的早期阶段,检测粪便DNA中p16基因的甲基化状态有望成为CRC早期无创诊断及CRC高风险人群筛查诊断的新途径。
Objective To evaluate the possibility of p16 gene methylation change in stool for screening early colorectal cancer. Methods Th DNA was isolated from fecal samples from 61 patients with colorectal cancer,21 patients with Adenoma and 20 normal volunteer,the methylation status of p16 gene was analyzed with Nest methylation-specific polymerase chain reaction （ nMSP ）. Results Methylated. p16. gene. occurred, in. 77.0% （47/61） ,59.3%（16/27）,5.0%（1/20） of patients with colorectal cancer,adenomas and nomal. The sensitivity and specificity of p16 gene methylation assay for colorectal cancer was 71.6% And 95.0% ,The p16 gene methylation-positive rate of the colorectal cancer had no significant relationship, with the age,sex and tumor site of the colorectal cancer patients. But the p16 gene methylationpositive rate of the coloreetal cancer had significant relationship, with the Dukes staging of the tumor,the degree of differentiation and lymph node metastasis. Conclusion The p16 gene methylation occurred in the early stages of colorectal cancer. Analysis of fecal p16 gene methylation is expected to serve as a new early detection way for eolorectal cancer or a new screening tool for individuals at high-risk of developing colorectal cancer.
出处
《结直肠肛门外科》
2009年第3期144-148,共5页
Journal of Colorectal & Anal Surgery
关键词
CRC
粪便
P16基因
甲基化
Colorectal cancer
Stool
P16 gene
Methylation
