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国外引进优良绣球的快繁技术研究 被引量:4

Study of Rapid Propagation Technology of the Introduced Fine Hydrangea L
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摘要 以国外引进优良绣球为材料进行扦插和组培快繁研究。扦插快繁用珍珠岩、蛭石、泥炭按不同比例设计7个处理,在平均温度22℃条件下,用半木质化枝条进行绣球扦插,统计插穗的生根率、生根条数和生根长度。结果表明:各处理间绣球的扦插生根率均无显著性差异;扦插基质中含泥炭的与不含泥炭的平均生根条数差异显著;处理中以珍珠岩∶蛭石∶泥炭=1∶1∶1的比例根最长,与其他处理间差异显著。组织培养快繁用带腋芽茎段为外植体,研究了不同浓度6-BA和NAA对绣球芽诱导增殖及生根的影响。结果表明:MS+6-BA 0.5 mg/L+NAA0.2 mg/L有利于绣球芽的诱导;MS+6-BA 2.0 mg/L+NAA 0.1 mg/L有利于芽增殖;MS+6-BA 0.5 mg/L+NAA 0.2 mg/L有利于生根。 The fine Hydrangea L species introduced from fremdness was used for cutting and tissue culture for rapid propagation. Semi lignified tresses were cuttinged separately under seven treatments with different proportion of perlite, vermiculite and turf, at 22 ~C temperature. The results indicated that there was no evident difference in the rooting rate under all treatments, but the average root number had a significant difference between mediums contenting turf and no turf. Among all the mediums, the best for rooting length was used in the ratio 1 : 1 : 1 as perlite : vermiculite : turf. In tissue culture for rapid propagation experiment, stems with axillary bud as explant were used to study 6-BA and NAA effect on bud inducement and rooting. The results suggested that the mediums,MS+6-BA 0. 5 mg/L+NAA 0. 2 mg/L, MS+6-BA 2. 0 mg/L+NAA 0. 1 mg/L and MS+6-BA 0. 5 mg/L+NAA 0. 2 mg/L,were suitable for bud inducement, proliferation and rootage respectively.
机构地区 德州学院农学系
出处 《北方园艺》 CAS 北大核心 2009年第7期192-194,共3页 Northern Horticulture
基金 山东省德州市科技发展计划项目(2006-67)
关键词 绣球 基质配比扦插 组培配方 快速繁殖 Hydrangea L Medium ingredient for cutting Culture medium direction Rapid propagation
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