摘要
目的：研究一氧化氮（ＮＯ）在防治烧伤早期心肌缺血缺氧性损害中的变化及意义。方法：动态观察大鼠３０％体表面积Ⅲ度烧伤后血浆及心脏ＮＯ、内皮素（ＥＴ）含量变化，应用外源性ＮＯ供体ＳＩＮ１以观察其对烧伤早期心脏损害的影响。结果：大鼠烧伤后心脏明显受损，烧伤后血浆及心肌组织ＮＯ－２、ＥＴ含量明显升高，由于ＮＯ－２升高幅度相对较低，ＥＴ／ＮＯ－２比值也显著增加；烧伤后在液体复苏下应用ＳＩＮ１可在一定程度上增加心肌组织超氧化物歧化酶（ＳＯＤ）、Ｎａ＋Ｋ＋ＡＴＰ酶活性及ＡＴＰ、ＡＤＰ含量，减少丙二醛（ＭＤＡ）、ＡＭＰ含量，减轻心肌组织水肿。结论：烧伤后由于心脏内源性ＮＯ的相对不足，导致心脏血管收缩，造成心肌组织缺血缺氧性损害，继而发生能量代谢紊乱及脂质过氧化损害；补充外源性ＮＯ则可在一定程度上逆转烧伤后心脏损害。
Objective:To elucidate the significance of nitric oxide (NO) in heart injury postburn.Methods:Rats were subjected to 30% TBSA fullthickness burns,dynamic changes in NO and endothelin (ET) contents were determined in plasma or myocardial tissue after burns.Meanwhile,the effect of SIN1,a kind of NO donor,on heart injury was observed in burned rats.Results:There was obvious heart damage in burned rats together with increases in NO-2 or ET levels in both plasma and myocardial tissue.The activities between ET and NO-2 were well balanced under the physiological state,however,the ratios of ET/NO-2 were significantly elevated following burns.Administration of SIN1 could increase superoxide dismutase and Na+K+ATPase activities,and ATP as well as ADP contents in myocardial tissue.Also,treatment with SIN1 had beneficial effects in reducing of severity of heart edema and ameliorating of heart damage to certain extent.Conclusions:During the early stage posburn,the pathological alteration in NO may contribute to the development of cardiac dysfunction due to vascular contraction and tissue hypoxia,which in turn inducing ATP depletion and lipoperoxidation damage in rats.
出处
《中国危重病急救医学》
CAS
CSCD
1998年第7期400-403,共4页
Chinese Critical Care Medicine
基金
国家自然科学基金
