摘要
目的：研究一氧化氮（ＮＯ）在烧伤早期肾脏损害中的变化及意义．方法：动态观察大鼠３０％Ⅲ度烧伤后血浆及肾脏ＮＯ、内皮素（ＥＴ）含量变化，应用外源性ＮＯ载体ＳＩＮ－１以观察其对烧伤早期肾脏损害的影响．结果：大鼠烧伤后肾脏明显受损，伤后血浆及肾组织ＮＯ、ＥＴ含量明显升高，由于ＮＯ升高幅度相对较低，ＥＴ／ＮＯ值也显著增加，肾组织ＥＴ／ＮＯ值改变与肾脏血流量的降低呈显著负相关；伤后在一定的液体复苏下应用ＳＩＮ－１可一定程度恢复肾脏血流量，增加肾组织超氧化物歧化酶（ＳＯＤ）、钠钾三磷酸腺苷（Ｎａ＋－Ｋ＋－ＡＴＰ）酶活性及三磷酸腺苷（ＡＴＰ）、二磷酸腺苷（ＡＤＰ）含量，减少丙二醛（ＭＤＡ）、腺苷酸（ＡＭＰ）含量，减轻肾组织水肿．结论：烧伤后由于肾脏内源性ＮＯ的相对不足，导致肾脏血管收缩，造成肾组织缺血缺氧性损害，继而发生能量代谢紊乱及脂质过氧化损害，外源性补充ＮＯ则可一定程度逆转烧伤后肾脏损害．
Aim: To elucidate the significance of nitric oxide (NO) in renal injury at the early stage of postburn. Methods: By use of a 30% TBSA (total body surface area) Ⅲ degree burnt rat model, we detected the dynamic changes of the contents of nitric oxide and endothelins in postburn plasma and kidney. Meanwhile, the effects of SIN 1, a kind of nitric oxide donor, were observed on renal injury in burnt rats. Results: There was obvious kidney injury in burnt rats, accompanied by the increase of nitric oxide or endothelins in plasma and kidney. The activities between endothelin (ET) and NO were well balanced in the physiological state. After burn, the ratio of ET/NO elevated significantly. Furthermore, the changes of the ratio of ET/NO were significantly correlated with the blood flow in kidney. Administration of SIN 1 was demonstrated to be able to prevent kidney from damage to some extent, by means of increment of renal blood flow, stimulation of the activity of SOD (superoxide dismutase) and Na + K + ATPase, enhancement of the contents of ATP and ADP in kidney, and reduction of the severity of renal edema. Conclusion: The pathological alteration of NO in the early stages of postburn in rats contributes to renal injury through vascular contraction and tissue hypoxia which in turn induces ATP depletion and lipoperoxidation damage.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
1997年第5期297-300,共4页
Chinese Journal of Trauma
基金
国家自然科学基金
