摘要
目的：探讨糖皮质激素（ＧＣ）快速抑制肾上腺髓质嗜铬细胞（ＡＭＣＣ）受刺激后分泌的机制。方法：用Ｆｕｒａ－２作Ｃａ２＋指示剂，观察ＧＣ对乙酰胆碱（ＡＣｈ）刺激引起的ＡＭＣＣ内游离钙（［Ｃａ２＋］ｉ）浓度升高的影响，并用放射免疫方法测定了ＧＣ对ＡＣｈ刺激后ＡＭＣＣ的环核苷酸含量的影响。结果：ＧＣ对ＡＣｈ引起的ＡＭＣＣ的［Ｃａ２＋］ｉ升高有快速抑制作用，但对ＡＣｈ刺激引起的环核苷酸含量升高无明显作用。
Objective: Primary cultures of chromaffin cells from rat adrenal medulla were used to study the intracellular mechanism of the rapid inhibitory effects of glucocorticoids on the stimulated secretion. Methods: Loading chromaffin cells with Fura2, a fluorescent indicator of free cytosolic Ca2+ concentration ([Ca2+]i). [Ca2+]i were measures with fluorescence spectrometer. The effect of corticosterone and dexamethasone on cAMP and cGMP accumulation in chromafin cells stimulated by acetylcholine for 20 min was examined with radioimmunoassay. Results: The data showed that dexamethasone significantly inhibited the elevation of [Ca2+]i response to acetylcholine. The effect of corticosterone and dexamethasone on cAMP and cGMP was not evident. Conclusion: [Ca2+]i may play an important role in the mechanism of the rapid effects of glucocorticoids on the secretion of chromaffin cells.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1998年第3期231-233,共3页
Academic Journal of Second Military Medical University
关键词
肾上腺髓质
嗜铬细胞
糖皮质激素
钙离子
adrenal medulla
chromaffin cells
glucocorticoids
calcium
cyclic nucleotides
