摘要
目的探讨肝星状细胞（ＨＳＣ）的活化与细胞间粘附分子１（ＩＣＡＭ１）表达的关系．方法用链酶蛋白酶和胶原酶原位灌流，Ｎｙｃｏｄｅｎｚ密度梯度离心分离大鼠ＨＳＣ，并进行体外培养，应用免疫组织化学方法观察静息或活化状态下的ＨＳＣ中ＩＣＡＭ１表达．结果静息的ＨＳＣ不表达ＩＣＡＭ１，而活化的ＨＳＣ表达ＩＣＡＭ１，且随着培养时间的延长ＩＣＡＭ１表达量逐渐增加．结论ＩＣＡＭ１表达与ＨＳＣ活化及肝纤维化的发生有关．
AIM To explore the relationship between activation of hepatic stellate cells (HSC) and expression of intercellular adhesion molecule 1(ICAM 1). METHODS HSCs were isolated and cultured from liver of Wistar rats by in situ perfusion with pronase and collagenase and density gradient centrifugation with Nycodenz. Expression of ICAM 1 in a quiescent and an activated HSC was detected by immunohistochemistry assay. RESULTS Expression of ICAM 1 was not observed in the quiescent HSC, but seen in the activated, and the amount of the expression was increasing with the time of culture. CONCLUSION Expression of ICAM 1 is related to activation of HSC and liver fibrosis.
基金
国家自然科学基金
关键词
星形细胞
肝硬化
病因
ICAM-1
liver/cytology
astrocytes
vascular cell adhesion
molecule 1/analysis
liver cirrhosis/etiology
