摘要
目的：检测慢性丙型肝炎患者外周血Ｔ细胞亚型和ＮＫ活性，并与正常人比较，以观察ＨＣＶ感染对患者细胞免疫功能的影响。方法：采用间接免疫荧光－流式细胞分析法检测外周血Ｔ细胞ＣＤ３、ＣＤ４、ＣＤ８表达，采用ＬＤＨ释放法测定ＮＫ活性。结果：慢性丙型肝炎患者外周血ＣＤ４、ＣＤ４／ＣＤ８比值、ＮＫ活性明显降低，与正常人比较差异有显著性（Ｐ值分别＜０．０１，＜０．０１和＜０．０５），而ＣＤ８升高（Ｐ值＜０．０５）；ＨＣＶ－ＲＮＡ阳性组与ＨＣＶ－ＲＮＡ阴性组比较，ＣＤ４／ＣＤ８比值、ＮＫ活性进一步下降，差异有显著性（Ｐ值分别＜０．０５）。结论：慢性丙型肝炎患者存在细胞免疫功能紊乱，提高其细胞免疫功能可能有利于患者的愈后。
To observe the changes of peripheral blood lymphocyte subsets and NK activity in patients with chronic hepatitis C. In thirty-two patients with chronic hepatic C peripheral blood CD3, CD4,CD8 and NK activity were tested by using immune indirect fluorescein- flow cytometry and LDH release assay. Peripheral blood CD4, CD4/CD8 and NK activity in patients with chronic hepatic cwere lower than those in normal group (P<0.01,<0.01 and <0.05 respectively), while CDg was nigher (P<0.05); CD4/CD8 and NK activity were lower in patients with HCV viremia than in patients without HCV viremia (P<0.05). The function of peripheral blood lymphocyte was inhibited in patients with chronic hepatitis C.
出处
《镇江医学院学报》
1998年第2期159-160,共2页
Journal of Zhenjiang Medical College
关键词
丙型肝炎
免疫学
T细胞亚型
NK活性
headings hepatitis
type C/immunology
T-lymphocyte subset
NK activity
