摘要
目的研究一种特效保肝食品不同制备工艺对四氯化碳所致小鼠肝损伤的保护作用及不同剂型中维生素B1的含量测定,综合筛选出最合理的剂型。方法将70只小鼠分为6组,编号,分别用此种食品的四种剂型(滤出液,转化液,未转化液,粉末)及蒸馏水,按0.2ml/10g进行灌胃,(正常组不做任何处理)。30d后,注射四氯化碳,制造急性肝损伤模型,断食17h后摘眼球取血,测小鼠血清天冬氨酸转氨酶,丙氨酸转氨酶的活性。再以维生素B1为指标,用色谱法分别测量在粉末与匀浆中的含量进行比较。结果此种食品维生素B1含量丰富,且药理实验证明可以降低因四氯化碳所致急性肝损伤小鼠血清的清天冬氨酸转氨酶和氨酸转氨酶活性的升高,且以粉末组保肝效果最明显。结论此种食品对肝脏有保护作用,而且在实验工艺中粉末剂型最可取。
Objective Study on the different preparation of a drag which can protect the liver from injury, then measure the quantity of Vitamin B1 and choose the best forms. Methods 70 mice were divided into 6 groups, number, such foods are used in four forms （filtrate into fluid, not into fluid, Powder） and distilled water by gavage for 0. 2 ml/10 g（ normal group received no treatment）. 30 days later, inject the mice with carbon tetrachloride, make model of acute liver injury and turn down the food. 17 hours later, measure the activity of ALT and AST in blood. Measure the quantity of vitamin B1 use HPLC. Results The results of such foods rich in vitamin B1. Pharmacology experiment shows that this drag can reduce the increased activity of ALT an AST and the powder is the best. Conclusion Such food is efficient for liver protection.
出处
《中国实用医药》
2009年第19期23-24,共2页
China Practical Medicine
关键词
肝损伤
小鼠
保肝
工艺筛选
维生素B1
Liver injury
Mice
Liver protection
Process Screening
Vitamin B1
