摘要
目的探讨醋酸曲普瑞林对人子宫内膜腺癌HEC-1B细胞的抑制作用及其分子机制。方法体外培养人子宫内膜腺癌HEC-1B细胞,用不同浓度醋酸曲普瑞林(10-9,10-8,10-7,10-6,10-5mol/L)为实验组,0mol/L组(不含醋酸曲普瑞林的培养液)为对照组,分别和HEC-1B细胞作用24、48、72h,四甲基偶氮唑蓝(MTT)法检测细胞抑制率,绘制肿瘤细胞生长抑制率曲线。免疫组织化学染色法检测72h细胞中C-myc蛋白表达,反转录-聚合酶链反应(RT-PCR)检测72hC-mycmRNA的表达。结果①当醋酸曲普瑞林浓度为10-9mol/L时,HEC-1B细胞生长即受到抑制,抑制率为8.97%;随着浓度的增大,抑制率渐高,浓度为10-5mol/L时抑制率上升至34.12%,与对照组比较抑制率差异有统计学意义(P<0.05)。②浓度从10-9～10-5mol/L的醋酸曲普瑞林作用72h后,C-myc蛋白和C-mycmRNA表达均有不同程度的下降,与对照组相比差异有统计学意义(P<0.05)。结论醋酸曲普瑞林可以抑制HEC-1B细胞的生长,其机制可能是通过下调HEC-1B细胞C-myc蛋白的表达而实现。
Objective To explore the effects of triptorelin acetate on cell growth of endometrial adenocarcinoma HEC-1B cell and its molecular mechanisms. Methods HEC-1B cells were cultured in different concentration of DMEM medium with triptorelin acetate in 24, 48 and 72 h, divided into different groups：experimental groups （ 10^- 9,10^-8, 10^-7 , 10^-6 , 10^-5 mol/L） and control group （0 tool/L）. The inhibitory rate of HEC-1B cells was detected by MTT method and the tumor cell inhibitory curve was drawed . The expression of C-myc protein was detected by immunocytochemical method. The expression of C-myc mRNA was detected by RT-PCR. Results The growth of HEC-1B cells was inhibited at the concentration of 10^-9mol/L, and the inhibitory rate was 8.97%. The inhibitory rate increased in a close-dependent manner. When triptorelin acetate concentration was 10^-5 mol/L, the inhibitory rate increased to 34.12%. Compared with control group, the inhibitory rates were significantly higher in experimental groups （P〈0.05）. Compared with control group, the expression of C-myc protein and C-myc mRNA significantly decreased after 72 h （P〈0.05）. Conclusion Triptorelin acetate could significantly suppress the proliferative activity of human endometrial adenocareinoma HEC-1B cell in vitro which may be related to down- regulation of C-myc protein.
