摘要
目的研究胰腺癌患者外周血CIM—CD8-T细胞比例与IL-4、IFN-γ含量变化及其相互关系。方法分别应用流式细胞仪及ELISA法检测25例胰腺癌患者和45名健康人外周血中CD4-CD8-T细胞比例和IL-4、IFN-γ的含量。结果25例胰腺癌患者CD4-CD8-T细胞占CD3^＋T细胞的比例为（4．2±1．7）％，45名健康人CD4-CD8-T细胞占CD3^＋T细胞的比例为（6．3±2．6）％，两组之间差异有统计学意义（P〈0．01）。胰腺癌患者外周血中IL-4含量为（86．3±23．3）fg／L，健康对照组外周血中IL-4含量为（56．2±9．2）fg／L，两组之间差异有统计学意义（P〈0．01）。胰腺癌患者外周血中IFN-γ含量为（16．4±4．8）fg／L，健康对照组外周血中IFN-1含量为（27．4±3．8）fg／L，两组之间差异有统计学意义（P〈0．01）。胰腺癌患者手术后CD4-CD8-T细胞比例显著高于术前（P〈0．01）。胰腺癌患者外周血CD4-CD8-T细胞比例与IL_4含量呈负相关，与IFN-γ含量呈正相关；胰腺癌患者CD4-CD8-T细胞比例、IL4含量与肿瘤临床分期有关（P〈0．05），与肿瘤分化程度均无关（P〉0．05），IFN-γ含量与肿瘤临床分期和肿瘤分化程度均无关（P〉0．05）。结论CD4-CD8-T细胞在胰腺癌患者外周血中比例降低，CD4-CD8-T细胞可能通过影响IFN-γ的分泌参与胰腺癌的发生和发展。
Objective To investigate the relationship between CD4- CD8-T cells ratio and IL-4, IFN-γ, levels in the peripheral blood of patients with pancreatic carcinoma. Methods Flowcytometer was used to analyze the CD4 - CD8 - T cells ratio in the peripheral blood of patients with pancreatic carcinoma and the IL-4,IFN-γ levels were detected by ELISA. Results The ratio of CD4 - CD8 - T cell in CD3-T cell from 25 pancreatic carcinoma patients was （4.2±1.7 ） % , the ratio of CIM - CD8 - T cell in CD3^＋T cell from 45 healthy person was （ 6. 3±2. 6） %, there was significant deviation between the two groups （ P 〈 0.01 ）. The IL-4 level of 25 pancreatic carcinoma patients was （ 86. 3±23.3 ） fg/L, the IL-4 level of 45 healthy person was （56. 2±9. 2） fg/L,there was significant deviation between the two groups（P 〈0. 01 ）. The IFN-γ level of 25 pancreatic carcinoma patients was （16. 4±4. 8） fg/L before operation, the IFN-γ level of 45 healthy person was （27.4±3.8） fg/L,there was significant deviation between the two groups（P 〈0. 01 ）. The ratio of CD4- CD8-T cell in pancreatic carcinoma patient after operation was higher than before operation. It could be found negative correlation between CIM - CD8 - T cells ratio and IL-4 level in pancreatic carcinoma patient,it could also be found positive correlation between CD4- CD8-T cells ratio and IFN-γ level in pancreatic carcinoma patient. In pancreatic carcinoma patient, the CD4- CD8 -T cells ratio and IL-4 level was significant associated with clinical stage （ P 〈 0. 05 ）, but no relationship with hisological differentiation （P〉0. 05）, it could be found no relationship between IFN-γ level and clinical stage, hisological differentiation （P 〉 O. 05）. Conclusion The CIM - CD8 - T cells ratio in the peripheral blood of patients is decreased, it may be participate in the carcinogenesis and development of pancreatic carcinoma by influence the IFN-γ levels.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2009年第13期995-998,共4页
Chinese Journal of Surgery
基金
安徽省国际科技合作基金资助项目（06088015）
安徽省高校省级自然科学研究项目省级重点资助项目（KJ2008A164）
