摘要
目的观察脑缺血再灌注时鼠脑纹状体区细胞外液（ＥＣＦ）多巴胺（ＤＡ）、去甲肾上腺素（ＮＥ）、５－羟色胺（５－ＨＴ）及其代谢产物高香草酸（ＨＶＡ）和５－羟吲哚乙酸（５－ＨＩＡＡ）的动态变化。方法采用脑内微透析技术，用高压液相色谱测定了前脑缺血３０ｍｉｎ、再灌注１２０ｍｉｎ时ＥＣＦＤＡ、ＮＥ和５－ＨＴ的变化。结果脑缺血１０ｍｉｎ时ＥＣＦＤＡ、ＮＥ迅速升高为缺血前的２８２和９１４倍，持续整个缺血期，再灌注后迅速下降达缺血前水平。脑缺血期ＨＶＡ、５－ＨＩＡＡ迅速下降，在缺血３０ｍｉｎ时达缺血前的４５％和５２％，再灌注后升高并在再灌注后６０ｍｉｎ，９０ｍｉｎ达缺血前的１５０％、１１３％。
Objective This study was undertaken to examine the patterns of changes in extracellular fluid (ECF) dopamine (DA), norepinephrine (NE), serotonin (5-HT) and their metabolites, homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA) in the striatum with transient forebrain ischemia. Methods Microdialysis was used to sample the brains extracellular space during 30 min forebrain ischemia followed by 120 min reperfusion. The microdialysate was measured by high performed liquid chromatography with electrochemical detection. Results ECF, DA, NE levels were increased by 282 and 9.14 fold of the basal levels respectivety, and reached their maximal values after 10 min ischemia. During ischemia ECF 5-HIAA and HVA decreased to 45% and 52% of preischemic levels. Upon reperfusion, ECF HVA and 5-HIAA immediately rise and reached a maximum of 150% and 113% preischemic levels. Conclusions DA, NE and 5-HT dysfunction was involved in ischemic neuronal damage.
出处
《中国神经精神疾病杂志》
CAS
CSCD
北大核心
1998年第4期196-198,共3页
Chinese Journal of Nervous and Mental Diseases
基金
全军九五攻关课题
关键词
脑缺血
单胺类神经介质
细胞外液
微透析
Cerebral ischemia
Monoamine neurotraasmitters
Extracellular fluid Microdialysis
