摘要
目的:研究凋亡抑制蛋白Livin,Survivin在肝癌细胞株HepG2的表达及5-Fu对肝癌细胞内凋亡抑制蛋白Livin,Survivin的诱导作用,探讨Livin,Survivin在肝癌化疗抵抗中的作用机制。方法:培养人肝癌细胞株HepG2,加入低浓度的化疗药物5-Fu,分别在加药前、加药后24h和48h采用逆转录聚合酶链反应(RT-PCR)和免疫印迹技术(Western-blot)检测livin,Survivin mRNA和蛋白的表达。结果:给予低浓度的5-Fu可诱导Livin,SurvivinmRNA和蛋白的表达发生变化,其中Livin mRNA在加药后24h和48h分别较加药前提高了0.6倍和1.1倍,Livin蛋白在加药后24h和48h分别较加药前提高了1.3倍和2.7倍,Survivin mRNA在加药后24h和48h分别较未加药提高了1.5倍和2.2倍,Survivin蛋白在加药后24h和48h分别较未加药提高了0.9倍和1.9倍。结论:Livin,Survivin在HepG2中有表达。5-Fu可诱导肝癌细胞内livin,Survivin的表达增加,抵抗化疗药物诱导的细胞凋亡。值得进一步研究以评价5-Fu对肝癌治疗的实用价值。
Objective：To investigate the expression of livin and survivin induced by 5-Fu agent,and determine the role of livin and survivin in anti-chemotherapy of hepatocellular carcinoma （HCC）. Methods：Low concentration of 5-Fu was added in cultured HepG2 cells. The expression of livin and survivin was detected by RT-PCR and western-blot on 24h and 48h after 5-Fu treatment.Results：Livin and survivin mRNA leve I was increased by 0.6 , 1.1 and 1.5,2.2 times, respectively.and thant of the protein expression was increased by 1.3 , 2.7 and 0.9, 1.9 times ,respectively.Conclusion：livin and survivin was expression in HepG2.A high expression level of livin and survivin was induced in HepG2 by 5-Fu,which reduced to apoptosis by 5-Fu. It＇s valuable to study the curative effect of 5-Fu to hepatocelluar carcinoma further.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2009年第7期849-851,共3页
Journal of Chongqing Medical University
