摘要
目的观察三磷酸肌醇（IP3）和Survivin基因表达变化在五羟黄酮（quercetin）治疗裸鼠移植入肝癌中的作用。方法以裸鼠移植入肝癌为对照，对照组腹腔注入含0．04％DMSO的RPMI 1640培养基0．05ml／g，Quercetin治疗组腹腔注入quercetin每日50mg／kg体重，3周后观察肝癌增长情况，3周后观察肝癌增长情况，并应用同位素试剂盒检测肝癌组织IP3含量，RT—PCR分析癌组织Survivin mRNA表达，Western blot分析肝癌组织Survivin蛋白表达。结果治疗组肝癌体积和重量均显著低于对照组[体积（15．8±10．1）mm^3比（52．3±26．5）mm^3，重量（44．8±10．4）mg比（91．3±31．4）mg]，IP3含量显著低于对照组[（13．4±1．4）pmol／mg protein比（35．3±6．6）pmol／mg protein]，Survivin mRNA表达低于对照组但差异无统计学意义[RI（灰度与面积之积的相对强度）0．60±0．06比0．79±0．19]（P〉0．05），Survivin蛋白表达显著低于对照组[RI（灰度与面积之积的相对强度）1．23±0．25比6．69±1．80]。结论Quercetin能减少IP3生成，下调肝癌组织Survivin基因表达，抑制裸鼠移植入肝癌增长。
Objective To investigate the implication of 1,4,5 - trisphosphate inositol （IP3） and Survivin gene expression in the treatment of hepatocellular carcinoma of nude mice by quercetin. Methods Animals with hepatocellular carcinoma were treated with quercetin 1 mg/（kgoday） （i. p） for 3 weeks. The volume and weight of tumaors were measured, and IP3, Survivin mRNA and Survivin protein were assayed by IP3 -[ ^3H ] Birtrak assay, RT-PCR and Western blotting, respectively. Results The tumor volume and weight of animals treated with quercetin were less than controls [ Volume （5.8 ± 10.1 ） mm^3 vs （52.3 ±26.5） mm^3 ; weight （44.8 ± 10.4） mg vs （91.3 ± 31.4） mg], IP3 continent was lower than that of controls [ （ 13.4 ± 1.4） pmol/mg protein vs （ 35.3 ± 6.6 ） pmol/mg protein ] , and the Survivin mRNA expression was lower in the group treated with quercetin than that of controls but had no significant difference [ RI 0.60 ± 0.06 vs 0.79 ± 0. 19 ] （ P 〉 0.05 ）. Survivin mRNA expression was lower in the group treated with quercetin than that of controls （ 1.23 ± 0.25 vs 6.69 ± 1.80）. Conclusion Quercetin can inhibit the growth of hepatocellular carcinoma tansplanted into liver of nude mice by reducing IP3 production and down-regulating Survivin gene expression.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2008年第11期1371-1373,共3页
Chinese Journal of Experimental Surgery
