摘要
以2,6-二(3-甲基-1-H-吡唑基)-4-溴吡啶为原料,经重氮化、溴化合成了新型时间分辨荧光免疫分析(TR-FIA)双功能螯合剂中间体2,6-二(3′-溴甲基-1′-吡唑基)-4-溴吡啶,通过IR、GC-MS1、HNMR和元素分析等对其结构进行了确认,探讨了合成条件及反应机理。同时,通过GC-MS1、HNMR和元素分析等对第一副产物4-溴-2-(3′-溴甲基-1′-吡唑基)-6-(3′-甲基-1′-吡唑基)吡啶的结构也进行了确认,以其为原料可继续合成目标化合物,大幅提高总产率。
A new bifunctional chelate intermediate of time-resolved fluorescence immunoassay 4-bromo-2,6-bis(3-(bromomethyl)-1H-pyrazol-1-yl)pyridine was prepared from 4-bromo-2,6-bis(3-methyl-lH-pyrazol-1-yl)pyridine by diazotizing reaction and brominating reaction.The structure of the product was determined by IR,^1 HNMR,GC-MS and elemental analysis.The reaction conditions and reaction mechanism were also researched.At the same time,the main outgrowth 4-bromo-2-(3-(bromomethyl)-lH-pyrazol-1-yl)-6-(3-methyl-1H-pyrazol-1-yl)pyridine was separated and its structure was determined by^1HNMR,GC-MS and elemental analysis as well.And the target product could also be prepared from it,so the yield was increased greatly.
作者
刘婷婷
宁志刚
赫文龙
潘利华
LIU Ting-ting;NING Zhi-gang;HE Wen-long;PAN Li-hua(Institute of Bioengineering,Changchun University of Technology,Changchun 130012,China;National Analytical Research Center of Electrochemistry and Spectroscopy,Changchun Instituteof Applied Chemistry,Chinese Academy of Science,Changchun 130022,China)
出处
《化学与生物工程》
CAS
2009年第6期40-42,共3页
Chemistry & Bioengineering
基金
国家自然科学基金资助项目(30471606)
