摘要
以IL-10的功能短肽(35肽,即IL-10第23号至第57号氨基酸)为导向部分与PE40(绿脓杆菌外毒素A除去受体结合区后的剩余部分)融合构建了IL-1023-57-PE40免疫毒素,对纯化的IL-1023-57-PE40进行了细胞毒活性测定、细胞ELISA、荧光标记和动物模型实体瘤组织切片实验,结果表明:构建的IL-1023-57-PE40符合免疫毒素的作用机制。实验改变了传统免疫毒素机制分析的方法,为免疫毒素的毒性机制分析做了一定有益的探索。
This immunotoxin IL-1023-57 PE/10 was constructed as a fusion protein hy using the funetional short peptide of IL-10 （35 peptide or the 23～57 amino acid residues） as the targeting part and PE40 （the remaining part of exotovin A of Pseudomonas aeruginosa after removing the rcceptor-binding region）. The cototoxieity activities of of this immunotoxin were assayed hy using cytological examination, cytotoxic assay, cell-ELISA, fluorescene-marker testing and animal model with peritonic tumor experiment. In the prsent study, it was proved that the constructed immunotoxin IL-1023-57-PE40 showed the biological characteristies of the specific immnnotoxin, and this method of analysis for eytotoxie mechanisms may provide a new way for the further studies.
出处
《现代免疫学》
CAS
CSCD
北大核心
2006年第3期243-246,共4页
Current Immunology
