摘要
采用分别测定己糖和戊糖含量的方法对DNS比色法进行了改进。结果表明:采用改进DNS比色法测定玉米芯水解液中总糖含量的回收率在98.90%～100.81%,RSD=0.62%,可准确、快速、简便地测定玉米芯水解液中的混合总糖含量。
The measuring precision of pentoses or hexoses by DNS method was well, but the measuring error of mixing total sugar of pentoses and hexoses was notable. Therefore, 3, 5-Dinitrosalicyclic acid method was modified by measuring of pentoses and hexoses respectively. The result showed the recollection rates of total sugar of corncob hydrolysate were in the range of 98.90% - 100.81%, the relative standard deviation was 0.62% and the precision in determination of mix- ing total sugar was raised obviously. So the method was accurate, fast and convenient and it was fit to determine total sugar in corncob hydrolysate in quantitation.
出处
《太阳能学报》
EI
CAS
CSCD
北大核心
2009年第6期820-823,共4页
Acta Energiae Solaris Sinica
基金
国家高技术研究发展计划(863)项目(2006AA02Z235)
国家自然科学基金(20606017)
国家自然科学基金重点项目(20336010)
江苏省高校自然科学研究项目(05KJB180043)
关键词
玉米芯
总糖
改进DNS比色法
定量测定
corncob
total sugar
modified DNS method
quantitative determination
