摘要
目的建立检测HLA-B27 mRNA表达水平的相对定量的方法。方法筛选未经治疗的AS患者外周静脉血27例,其中HLA-B27阳性患者22例,HLA B27阴性患者5例,对标本进行Total RNA提取,然后用RT-PCR方法进行HLA-B27反转录及相对定量。结果22例HLA-B27阳性AS患者有着相对较高的HLA-B27 mRNA表达水平,最低值为1.16,最高值为37.01,均值为7.77。5例HLA-B27阴性AS患者均值为1.19,接近于HLA-B27阴性健康对照组。结论检测外周静脉血HLA-B27mRNA表达水平对检测AS是一种有效可行的方法。
Objective To establish a method for relatively quantitative measurement of HLA- B27 mRNA. Methods Totally 27 cases of peripheral venous blood samples were collected from patients with ankylosing spondylitis, including 22 cases from the patients with HLA-B27 positive and 5 cases with HLA-B27 negative. Total RNA from the venous blood samples was extracted, and then the expression level of HLA-B27 mRNA was quantitatively detected by using reverse transcription polymerase chain reaction （RT-PCR）. Results The expression level of HLA-B27 mRNA was higher in patients with HLA-B27 positive （n=22; 1.16-37.01, mean value was 7.77） than that in patients with HLA-B27 negative （n= 5; mean value was 1.19, close to that of healthy control group）. Conclusion Relatively quantitative measurement of HLA-B27 mRNA is helpful for monitoring of ankylosing spondylitis.
出处
《国际检验医学杂志》
CAS
2009年第6期535-537,共3页
International Journal of Laboratory Medicine
