摘要
目的探讨电针（electroacupuncture,EA）对慢性应激海马神经元型一氧化氮合酶（nNOS）表达的影响。方法30只雌性SD大鼠随机分成3组：模型组、电针组和对照组。每组10只。模型组大鼠连续接受21d各种不同的应激，平均每种应激使用2～3次。电针组模型建立后中止刺激，给予电针“三阴交”穴和“百会”穴．连续电针21d。对照组不给予刺激和电针。第42天将3组大鼠处死取脑。利用免疫组织化学方法检测海马区nNOS的表达情况。结果模型组大鼠海马nNOS阳性神经元的数量减少（P〈0．05），染色变淡；电针后海马nNOS阳性神经元的数量有所增加（P〈0．05）。结论电针可上调nNOS在大鼠海马内的表达。
Objective To observe the effects bf electroacpuncture（EA） on the expression of neuronal nitric oxide synthase（nNOS） in hippocampus of the chronic stress rats. Methods 30 female rats were randomized into 3 groups（model group, EA group and control group）, each group including 10 rats. The rats of both model group and EA group were given various kinds of stimulation for 21 days, then the rats of EA group were given EA treatment in the following 21 days. Rats of 3 groups were killed to obtain their brains after 42 days, the expression of nNOS in hippocampus were detected using immunohistochemical method. Results The number of nNOS of the model group decreased significantly in hippocampus compared with the control group（P〈0.05）,while the number of nNOS of EA group were higher than that of model group（P〈0.05）. Conclusion EA could upregulate the expression of nNOS in hippocampus of rats.
出处
《四川解剖学杂志》
2009年第2期3-4,22,共3页
Sichuan Journal of Anatomy
基金
安徽省教育厅科研项目(2006KJ111C)
关键词
应激
海马
电针
一氧化氮合酶
大鼠
Stress
Hippocampus
Electroacupuncture
Nitric oxide synthase
Rats
