摘要
采用硫酸铵沉淀法获得鼠李糖乳杆菌B10发酵液中水溶蛋白粗提物,并进行葡聚糖凝胶SephadexG-100柱纯化,分离得到不同的蛋白组分峰Ⅰ、峰Ⅱ和峰Ⅲ,分别对各组分DPPH自由基清除能力、清除超氧自由基(O2-·)和羟自由基(·OH)的能力及超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性进行分析。结果表明,纯化前后各样品均对DPPH·有清除作用,其中纯化后的峰Ⅰ对DPPH·清除能力最强,峰Ⅱ、峰Ⅲ次之,均高于水溶蛋白粗提物;纯化后的峰Ⅰ对O2-·和·OH的清除能力最强,均高于5mmol/L的Vc溶液和水溶蛋白粗提物对O2-·和·OH的清除作用,峰Ⅱ和峰Ⅲ均未检测到对O-·和·OH的清除能力。
In this paper, ammonium sulfate precipitation method was used to obtain the soluble protein from Lactobacillus rhamnosus B10 broth. The different protein components： the peak I, peak II and peak III. It were purified and isolated using dextran gel Sephadex G-100 column. Their scavenging capacity of DPPH., superoxide radicals （O2＂） and hydroxyl radical （.OH） were analysed. The results showed that the soluble protein, peak I, peak II and peak III has scavenging effect on DPPH., and the peak I has more DPPH- scavenging capacity than peakII, III, the soluble protein is less than peak II, III. The O2- and .OH scavenging capacity of the peak I is the strongest, and it was higher than 5 mmol/L Vc solution. The O2 and .OH scavenging capacity of the soluble protein is weak.
出处
《食品科技》
CAS
北大核心
2012年第8期235-239,共5页
Food Science and Technology
基金
国家青年科学基金项目(31101252)
吉林省科技引导计划项目(20090576)
关键词
鼠李糖乳杆菌
水溶蛋白
抗氧化
Lactobacillus rhamnosus
soluble protein
antioxidant activity
