摘要
利用A-PAGE的醇溶蛋白标记,对来自亚洲和北美的86份老芒麦种质的遗传多样性和遗传关系进行了分析。电泳共检测到52条醇溶蛋白条带,其中47条为多态性条带,多态性带百分比达90.4%。种质间遗传相似系数的变幅为0.108-0.952,平均值为0.373。利用Shannon多样性指数反映了类似的结果,即供试种质间的多样性指数达到0.460的较高水平。基于多样性指数计算了老芒麦种质地理类群遗传分化程度,地理类群内和类群间的遗传变异分别占总变异的55.8%和44.2%,这表明种质间存在较高水平的遗传多样性。对供试种质和地理类群的聚类分析结果均显示,来源于青藏高原的种质与其他地理来源的种质具有较大的差异,可以分成明显的2支。这种聚类结果可能与老芒麦种质的地理来源和生态适应性有关。本研究结果可为老芒麦种质的收集保护及核心种质构建提供有益信息。
Gliadin among eighty-six scored, of which markers based on A-PAGE were used to analyze genetic diversity Elymus sibiricus accessions from Asia and North America. A total 47 were polymorphic （90. 4%）. The generated similarity matrix and genetic relationships of 52 gliadin bands were showed that the genetic diversity within the accessions was fairly high （average similarity index=0. 373）. Similarity values among the accessions ranged from 0. 108 and 0. 952. Similar results were obtained when genetic diversity was estimated using the Shannon index of diversity. The total genetic diversity across all accessions was 0. 460. The proportion of variation explained by within geographic groups and between geographic groups diversity was 0. 559 and 0.442, respectively. Cluster analysis showed a clear demarcation between accessions from the Qinghai-Tibetan Plateau, China and the others as separate groups. The extent of genetic diversity and genetic relationship among accessions is discussed. The clustering pattern probably depended on geographic origin and ecological adaptability of the accessions.
出处
《草业学报》
CSCD
北大核心
2009年第3期59-66,共8页
Acta Prataculturae Sinica
基金
四川省"十一五"牧草育种攻关项目
国家重点基础研究发展计划(973计划)项目(2007CB108907)资助
关键词
老芒麦
醇溶蛋白
遗传多样性
地理类群
遗传分化
Elymus sibiricus
gliadin
genetic diversity
geographic groups
genetic differentiation
