摘要
利用原位脱氧核糖核酸末端转移酶标记技术和ＳＰ免疫组化方法检测了肝细胞癌和癌旁肝组织中细胞凋亡和Ｃ－ｍｙｃ蛋白的表达。结果显示，癌组织中凋亡细胞密度明显低于癌旁组织（Ｐ＜０．０１）；ｃ－ｍｙｃ蛋白在癌组织和癌旁肝组织中的阳性率分别为４０％（１２／３０）和８６．７％（２６／３０），差异有显著性，其表达强度亦以癌旁肝组织为强（Ｐ＜０．０ｌ）。结果提示，ｃ－ｍｙｃ蛋白的表达既可诱导细胞凋亡又可促进细胞增殖；进一步支持癌旁肝细胞可能系具有肿瘤性增殖潜能的癌前细胞群的观点。
Apoptosis and expression of c-myc protein were detected by in situ deoxynu-cleotide transferase labelling technique and SP immunohistochemical method in hepa-tocellular carcinoma (HCC)and their surrounding liver tissue.The results showedthat the density of apoptic cells in HCC was lower than that in pericarcinomatousliver tissue(P<0.01).The positive rates of c-myc protein were 40% (12/30)and 86.7%(26/30)in HCC and their surrounding liver tissue respectively,therewas significant difference between them,The expressive in tensity of c-myc proteinin pericarcinomatous liver tissue was stronger than that in HCC (P<0.01).Theresults suggest that expression of c-myc protein may induce apoptosis and also causeproliferation of hepatocytes and further suport the view that pericarcinomatous hep-atocytes may possess latent capacity of tumorous prolifeation.
出处
《湖南医学》
1998年第3期132-133,共2页
Hunan Medical Journal
关键词
肝肿瘤
肝细胞癌
C-MYC
细胞凋亡
liver neoplasms carcinoma apoptosis immunohistochemistry
