摘要
为了探讨肿瘤化疗机理，作者采用流式细胞术单参数分析方法，检测了４５例恶性肿瘤患者化疗前后外周血细胞的凋亡（Ａｐｏ）、Ｓ期细胞比率（ＳＰＦ）和增殖指数（ＰＩ）的变化。结果表明，肿瘤患者血细胞Ａｐｏ水平明显高于正常对照（Ｐ＜０．０１），化疗结束时进一步升高（Ｐ＜０．０１），化疗后１个月又恢复到化疗前的水平。肿瘤患者的ＳＰＦ和ＰＩ均显著高于正常对照（Ｐ＜０．０１），化疗结束时明显减低，ＰＩ尤为显著（Ｐ＜０．０１），并持续到化疗后１个月。从而可见，化疗使患者血细胞Ａｐｏ呈“一过性”增高，肿瘤的化疗机制主要是化疗药物使细胞增殖活性减低，细胞聚积在Ｇｏ／Ｇ１期，同时药物诱导细胞凋亡增加。
In order to investigate the effective mechanism of anti tumor chemotherapy,Apo (apoptosis),SPF (S phase fraction) and PI (proliferation index) in PBM (peripheral blood monocytes) of 45 patients with malignant tumor were measured by flow cytometry. The results showed that the Apo levels in patients group were higher than that in normal control ( P <0.01),They were further increased after chemotherapy ( P <0.01) and at one month later returned to the previous level. Also, SPF and PI,in especial PI,in patients group were higher than that in normal control ( P <0.01). They were reduced after chemotherapy ( P < 0.01 ) and the reduced levels continued for at least one month. The data indicate that the cellular apoptosis and inhibited cellular proliferation may be a main mechanism of anti tumor chemotherapy.
出处
《实用癌症杂志》
1998年第2期118-120,共3页
The Practical Journal of Cancer
关键词
肿瘤
流式细胞术
细胞凋亡
药物疗法
Malignant tumor Flow cytometry Apoptosis Chemopherapy DNA content
