摘要
目的探讨被晚期糖基化终产物修饰的β２微球蛋白（ＡＧＥｓβ２Ｍ）与人类成纤维细胞的相互关系。方法用去垢剂提取人成纤维细胞系（ＨＳＦｓ）膜成份。用免疫印迹法和免疫荧光法鉴定细胞ＡＧＥ受体（ＲＡＧＥ）。用放射性配体结合法观察ＡＧＥβ２Ｍ与ＨＳＦｓ的相互关系。结果ＨＳＦｓ膜表面表达ＲＡＧＥ。ＡＧＥβ２Ｍ能以特异、剂量依赖的方式与ＨＳＦｓ结合［亲和常数（Ｋｄ）≈８６５ｎｍｏｌ／Ｌ］，这一过程可被抗ＲＡＧＥ抗体所抑制，提示该结合是由ＲＡＧＥ所介导。结论成纤维细胞及其相关的间质细胞可能是ＡＧＥβ２Ｍ病理生物学效应的靶细胞，从而可能参与了透析相关性淀粉样变（ＤＲＡ）的发生。
bjective An important component of amyloid fibrils in dialysisrelated amyloidosis (DRA) is a form of β2microglobulin modified with advanced glycation end products (AGEs) of the Maillard reaction, known as AGEβ2M. The aim of the study is to investigate the interaction between AGEβ2M and human fibroblasts. Methods Using polyclonal antibody to the receptor for AGEs(RAGE), the immunoreactive material on Western blots of detergent extracts from a human fibroblast cell line (HSF, GM05757A), and on surface of HSFs by immunofluorescence studies were detected. Binding assays were performed using the radiolabeled AGEβ2. Results RAGE was indentified on the surface of HSFs. 125[KG*2〗ⅠAGEβ2M bound to HSFs in a specifi0.c, dosedependent manner (Kd≈86.5 nmol/L), a process inhibited in the presence of antiRAGE IgG. Conclusion Human fibroblasts and related mesenchymal cells may be targets for the pathobiological action of AGEβ2M, and might be involved in the pathogenesis of DRA.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
1998年第2期84-88,共5页
Chinese Journal of Nephrology
基金
国家自然科学基金
关键词
AGES
Β2微球蛋白
成纤维细胞
淀粉样变
DRA
Receptor for advanced glycation end products β2 Microglobulin Fibroblast Dialysisrelated amyloidosis
