摘要
目的:多西紫杉醇是一种新型的半合成的抗癌药,对多种实体瘤细胞具有明显的细胞毒性和放疗增敏作用。文中探讨多西紫杉醇联合放疗诱导人宫颈癌细胞系Hela凋亡与其调节肿瘤坏死因子相关诱导凋亡配体(TRAIL)及其受体表达的相关性。方法:用Hoechst 33342染色法和原位细胞凋亡检测法分别检测单纯多西紫杉醇组、单纯放疗组以及多西紫杉醇联合放疗组Hela细胞凋亡情况,采用RT-PCR法检测3组细胞中TRAIL及其受体TRAIL-R mRNA表达变化。结果:与单纯多西紫杉醇组和单纯放疗组相比,多西紫杉醇联合放疗组中Hela凋亡细胞数明显增多,TRAIL及其受体TRIAL-R1、TRAIL-R2 mRNA表达明显升高。结论:多西紫杉醇联合放疗诱导Hela细胞凋亡的机制可能是通过上调TRAIL及其受体TRAIL-R1、TRAIL-R2 mRNA表达来实现的。
Objective： Docetaxel as a newly semi-synthesized anti-tumor drug, exhibits significant cyrotoxic activity and enhanced sensitivity for radiotherapy. The aim of this study was to investigate the correlation of the apoptosis of human Hela cell line with the expressions of TRAIL and its receptors induced by the combination therapy of Docetaxel with radiation. Methods： We treated the Hela line by Docetaxel, radiation, and combination therapy of Docetaxel with radiation, respectively, detected the apoptosis of the Hela cells using Hoeehst 33342 staining and the TUNEL method, and observed the changes in the mRNA expressions of TRAIL and its receptors TRAIL-R in the three groups by RT-PCR. Results ： Compared with the Docetaxel and the radiation groups, the apoptosis of the Hela cells was increased significantly in the combination therapy group, and so were the mRNA expressions of TRAIL and its receptors TRIAL-R1 and TRAIL-R2. Conclusion ： The combination therapy of Docetaxel with radiation increased the apoptosis of the Hela cell line, which might be mediated by the up-regulation of mRNA expressions of TRAIL and its receptors TRAIL-R1 and TRAIL-R2.
出处
《医学研究生学报》
CAS
2009年第5期460-463,I0003,共5页
Journal of Medical Postgraduates
基金
黑龙江省科技厅青年基金资助项目(批准号:QC06C065)
黑龙江省人事厅博士后基金资助项目(批准号:LBH-Z06252)
关键词
多西紫杉醇
宫颈癌
凋亡
TRIAL
TRIAL受体
Docetaxel
Hela cell
Apoptosis
TNF related apoptosis-inducing ligand
TNF related apoptosis-inducing ligand receptor
