摘要
目的对洛莫司汀脂质体进行质量评价，建立洛奠司汀脂质体包封率的测定方法。方法采用微柱离心法分离洛莫司汀脂质体与游离药物，采用HPLC法测定洛莫司汀含量。结果微柱离心法对洛莫司汀游离药的吸附率在97．25％～98．36％内，空白脂质体回收率在98．60％-100．5％内，洛莫司汀脂质体和游离洛莫司汀得到良好的分离；在选定色谱条件下，洛莫司汀与脂质体分离良好，辅料不干扰测定，洛莫司汀质量浓度在0．5—50．0mg·L^-1内与峰面积呈良好的线性关系（r=0．9999），日内和日间RSD均小于2％（n=5），加样回收率在97．96％-98．79％内。结论微柱离心-HPLC法可用于洛莫司汀脂质体包封率的测定。
Objective To evaluate the quality and determine the entrapment efficiency of lomustine lipo- somes. Methods The microcolumn centrifuge method was adopted to separate the free lomustine from lipo- some and HPLC method was used for analyzing the amount of lomustine. Results The interception to free lomustine by microcolumn centrifuge method was 97.25% -98.36%, the recovery rate of liposomes was 98. 60% - 100. 5% ,lomustine liposomes and lomustine could be well separated;A calibrated linear curve of lomustine was within 5.0 - 50. 0 mg.L^-1 （ r = 0. 999 9 ）, and RSDs within day and between days were all less than 2% （ n = 5 ）, the recovery rate of lomustine with blank liposomes was in a range of 97.96% - 98.79%. Conclusions The microcolunm centrifuge-HPLC method can be used for the determination of entrapment efficiency of lomustine liposomes.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2009年第6期447-450,共4页
Journal of Shenyang Pharmaceutical University
基金
国家自然科学基金资助项目(30672555)
