摘要
以淀粉水解液、纸浆为原料，分批添加底物制备纤维素酶，底物浓度为４０ｇ／Ｌ时，滤纸酶活力和纤维二糖酶活力分别达５１ＦＰＩＵ／ｍＬ和１５ＩＵ／ｍＬ，蛋白质浓度达２２９ｍｇ／ｍＬ；当底物浓度增加到７４ｇ／Ｌ时，滤纸酶活力几乎成正比例地提高到１４８ＦＰＩＵ／ｍＬ。以纸浆为原料分批添料产酶，添料速度以０３ｇ／ｄ较为适宜，底物浓度达７７ｇ／Ｌ时，滤纸酶活力达９９５ＦＰＩＵ／ｍＬ，酶产率和酶得率分别为３１８９ＦＰＩＵ／Ｌ·ｈ和１２９２ＦＰＩＵ／ｇ纤维素。通过分批添加底物，可实现在高浓度底物下制备纤维素酶而达到降低产酶成本的目的。
Starch hydrolysate and sulphite pulp have been examined as the candidate feedstocks for cellulase production in fedbatch cultures by a strain of Trichderma reesei. The filter paper activity, cellobiase activity and protein concentration were 5.1 FPIU/mL, 1.5 IU/mL and 2.29 mg/mL, respectively, when the substrate concentration was 40 g/L. The filter paper activity increased to 14.8 FPIU/mL as the substrate concentration was risen to 74 g/L. When sulphite pulp was used as the sole carbon source, the hightest filter paper activity, 9.95 FPIU/mL,was obtained with 77 g/L of pulp fed at a rate of 0.3 g/d, with the production and productivity being 31.89 FPIU/L·h and 129.2 FPIU/g cellulase, respectively. It was found that the cellulase production cost could be reduced by the technology of fedbatch cultures adopting high substrate concentration.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
1998年第2期73-77,共5页
Chemistry and Industry of Forest Products
