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不同固定液、染色法显示结缔组织肥大细胞效果的比较 被引量:7

CONNECTIVE TISSUE MAST CELLS: EVALUATION OF FIXATION AND STAINING TECHNIQUES
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摘要 本研究用6种固定液、4种染色法,观察对显示小鼠耳廓、舌和背皮肤结缔组织肥大细胞的影响。结果表明:一种固定液固定的组织,用不同的染色法染色或不同固定液固定的组织,用一种染色法染色,显示肥大细胞的效果不同。这可能是由于固定液能影响肥大细胞颗粒对染料的亲合力。本文对不同部位的结缔组织肥大细胞对固定液的反应有差异这一现象,进行了讨论。 In the current study, 6 fixtures and 4 staining methods were used to demonstrate the effect of fixation and staining on the manifestation of the connective tissue mast cells of mice.The results showed that the effects were different when stained with different staining methods or when fixed with different fixatives.This may be caused by the influence of fixatives changing the affinity of the granules of mast cells to the dyes.In the paper, it is also discussed that the reaction of mast cells in variant places to fixation is different.
出处 《解剖学杂志》 CAS CSCD 北大核心 1990年第4期287-291,共5页 Chinese Journal of Anatomy
关键词 CTMC 固定 染色 Connective Tissue Mast Cells, Fixation, Staining.
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参考文献2

  • 1Urban Wingren,Lennart Enerb?ck. Mucosal mast cells of the rat intestine: a re-evaluation of fixation and staining properties, with special reference to protein blocking and solubility of the granular glycosaminoglycan[J] 1983,The Histochemical Journal(6):571~582
  • 2Graham Mayrhofer. Fixation and staining of granules in mucosal mast cells and intraepithelial lymphocytes in the rat jejunum, with special reference to the relationship between the acid glycosaminoglycans in the two cell types[J] 1980,The Histochemical Journal(5):513~526

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