摘要
本文报道人胃低分化腺癌细胞(MGC-803)在外源性三磷酸腺苷(ATP)的连续作用下,细胞增殖受到明显的阻抑,4天以后细胞增殖的抑制率达80％以上,细胞内的骨架系统有较明显的改善。微管由中央区的微管组织中心一直伸展到细胞边缘附近,使细胞充分铺展并变扁平,近似于正常上皮细胞的形态。微丝束变粗壮,纵横交错,布满细胞周边的细胞质内。点状的肌动蛋白荧光明显减少。细胞内外的纤维粘连蛋白的荧光反应更加明显。本文并讨论了细胞增殖和细胞骨架系统之间的关系。
This study was carried out to investigate the effects of ATP on cultured human stomach carcinoma MGC-803 cells. After ATP(0.16—0.23 mg/ml, 24—120 hrs)treatment, inhibition of cell growth rate was observed. The inhibition rate reached 80% after 96 hr exposure to ATP. At the same time, anti-tubulin immunofluorescent staining showed increase of organized microtubules (MT) in ATP-treated MGC-803 cells, in contrast with the poor MT immunofluorescence in control MGC-803 cells. By using rhodamie phalloidin combined staining, increased number of actin bundles, took the place of actin dots and patches which were seen in control MGC-803 cells. Immunofluorescent staining of fibronectin in ATP-treated MGC-803 cells was also increased evidently as compared with the control MGC-803 cells. Concomitant with the improvement of MT and stress fiber organization, the cell Concohas become more flattened. It appeared that the increase of MT and stress fibers was closely related to the recovery of cell morphology in ATP treated tumor cells. It is therefore concluded that ATP not only may inhibit tumor cell proliferation, but also may induce differentiation of cell morphology of tumor cells through its action on cytoskeletal structures.
出处
《解剖学报》
CAS
CSCD
北大核心
1990年第3期274-278,共5页
Acta Anatomica Sinica
基金
国家自然科学基金
关键词
三磷酸腺苷
胃腺癌细胞
细胞增殖
Cell proliferation
Adenosine triphosphate (ATP)
Human stomach carcinoma cell line MGC-803
Microtubule
Microfilament bundle
Fibronectin
Immunofluorescence
