摘要
目的:观察低氧(Hypoxia,Hyp)对大鼠视网膜神经节转化细胞(retinaganglioncell-5,RGC-5)氧化应激损伤的影响及牛磺酸(Taurine,Tau)的防护效应。方法:将RGC-5置于低氧条件(5%O2,5%CO2,90%N)2,加入不同浓度的牛磺酸(0.05mM、0.1mM、0.5mM、1mM)预处理后培养12h,24h和48h,使用MTT法检测细胞活力,并通过对NO、GSH、MDA等指标的检测,观察牛磺酸对RGC-5的保护效应。结果:低氧处理后RGC-5细胞活力明显降低(P<0.05),牛磺酸处理组细胞活力明显高于低氧组,其中0.1mM牛磺酸组作用最为显著(P<0.05);低氧组与常氧组比较,RGC-5的NO、GSH含量明显降低(P<0.05),而MDA含量显著升高(P<0.05);牛磺酸处理组与低氧组比较,RGC-5细胞GSH,NO的含量显著升高(P<0.05),而MDA的含量显著降低(P<0.05)。结论:牛磺酸能有效增强低氧损伤中RGC-5细胞的活力,其机制可能与牛磺酸可以提高其抗氧化能力有关。
Objective： To investigate the effects of hypoxia induced oxidative stress damage to transformed rat retinal ganglion cells （RGC-5）and further explore the protective effects oftaurine. Methods： Pretreated taurine （0.05mM, 0.1mM, 0.5mM, 1mM）for 12h, 24h and 48h, RGC-5 cells were cultured in an hypoxia incubator （5% 02, 5% CO2, 90% N2）. Then determined the cell viability by the MTT method and tested the levels ofglutathione （GSH）, NO and malonaldehyde （MDA） in order to research the protective effect of taurine on RGC-5. Results： The cell viability of RGC-5 cells of the hypoxia group decreased apparently （P〈0.05）, but the cell viability in the taurine-treated hypoxia groups were higher than the hypoxia group （P〈0.05） and the most significant effect oftaurine was observed at 0.1 mM. The levels of GSH and NO in the hypoxia group were lower, while MDA was higher than those in control group （P〈0.05）. Compared with the hypoxia group, the levels of GSH and NO were prominently higher, but MDA was dramatically lower in the taurine-treated hypoxia group （0.1 mM ; P〈0.05）. Conclusion： Taurine can effectively reinforce the Viability of RGC-5 ceils under hypoxia, which may be related to its anti-oxidative effects.
出处
《现代生物医学进展》
CAS
2009年第11期2083-2086,共4页
Progress in Modern Biomedicine
基金
全军"十一五"专项资助项目(06z028)
重庆市营养与食品安全重点实验室开放课题(2007)
第三军医大学科研创新基金(2007XG19)
