摘要
目的:了解大肠癌组织中MAD2和p53表达概况,探寻其表达差异及临床意义。方法:通过实时荧光定量PCR和免疫组织化学方法检测大肠癌、腺瘤及正常大肠黏膜组织中MAD2表达,并对大肠癌组织中MAD2扩增产物进行测序。同时利用免疫组织化学方法检测大肠癌和正常组织中p53的表达。结果:大肠癌组织中MAD2的阳性表达明显高于腺瘤和正常大肠黏膜组织,三者比较差异有统计学意义,P<0.001。MAD2表达与分化程度和生存时间有关,P<0.05。大肠癌组织中未见MAD2基因突变。正常大肠黏膜组织中p53表达均为阴性,大肠癌组织中p53阳性表达率为50%。结论:MAD2和p53表达异常是大肠癌产生的机制之一,与大肠癌的发生、发展有关。
OBJECTIVE： To study the expression of gene MAD2 and p53 in colorectal cancer, and evaluate their difference and clinical significances. METHODS： Inmmunohistochemistry method and realtime fluorescence quantitative PCR were used to analyze the expression of gene MAD2 in the colorectal cancer, adenoma and normal tissue. PCR amplification and DNA sequencing were performed to detect the mutations of gene MAD2. p53 was determined in the colorectal cancer and normal tissue by inmmunohistochemistry method. RESULTS： The expression of MAD2 in colorectal cancer were significantly higher than that in adenoma and normal tissues. There were significant difference among colorectal cancers, adenoma and normal tissues, P〈0. 001, which were related to the tumor differentiation and survival time, P〈0.05. No mutation of gene MAD2 was found in colorectal cancer. No p53 protein expression was observed in the normal tissue. The rate of p53 positive expression in colorectal cancer was 50%. CONCLUSION： Positive expressions of MAD2 and p53 are a mechanism of tumor and they were involved in colorectal oncogenesis.
出处
《中华肿瘤防治杂志》
CAS
2009年第9期687-689,共3页
Chinese Journal of Cancer Prevention and Treatment
基金
南京军区医学科学研究"十一五"计划课题资助项目(06 MA39)
