摘要
目的了解大肠癌中MAD2表达情况,探讨其在大肠癌、腺瘤及正常大肠黏膜组织中的表达差异,分析其临床意义。方法通过实时荧光定量PCR和免疫组化方法检测大肠癌、腺瘤及正常大肠黏膜组织中MAD2表达,并对大肠癌组织中MAD2扩增产物进行测序。结果免疫组化方法结果显示大肠癌组织中MAD2的阳性表达明显高于腺瘤和正常大肠黏膜组织(66.7%vs39.6%vs22.9%),三者比较差异有统计学意义(P<0.001)。MAD2表达与肿瘤分化和无瘤生存时间有关(P<0.05)。实时荧光定量PCR提示肠癌组织中MAD2的表达量明显高于正常大肠黏膜组织,大肠癌组织中未见MAD2基因突变。结论基因MAD2表达异常是大肠癌产生的机制之一,与大肠癌的发生、发展有关,MAD2可能是大肠癌预后的一项重要预测指标。
Objective To study the expression of gene MAD2 in colorectal cancer and the relation between the expression of gene MAD2 and colorectal cancer,adenoma and normal tissue and to evaluate their clinical significances. Methods Immunohistochemistry method and real-time fluorescence quantitative PCR were used to analyze the expression of gene MAD2 in the colorectal cancer,adenoma and normal tissue.PCR amplification and DNA sequencing were performed to detect the mutations of gene MAD2. Results The expression of MAD2 in colorectal cancer was obviously higher than that in adenoma and normal tissue by inmmunohistochemistry method（66.7% vs 39.6% vs 22.9%）. There was significant difference among colorectal cancers,adenoma and normal tissue （P〈0.001）,and it was related to tumor differentiation and survival time （P〈0.05）. The quantity of MAD2 in colorectal cancer was obviously higher than that in normal tissue by real-time fluorescence quantitative PCR and no mutation of gene MAD2 was found in colorectal cancer. Conclusion Positive expression of MAD2 is mainly involved in colorectal carcinogenesis and MAD2 may be associated with prognosis of colorectal cancer.
出处
《实用肿瘤杂志》
CAS
北大核心
2009年第5期437-440,共4页
Journal of Practical Oncology
基金
南京军区医学科学研究"十一.五"课题项目(06MA39)
