摘要
目的检测青蒿素和青蒿琥酯对兔耳增生性瘢痕模型瘢痕防治的效果。方法用青蒿素和青蒿琥酯配制外用膏剂治疗兔耳腹侧增生性瘢痕模型，用药28d后切取瘢痕HE染色、VG染色，检测瘢痕组织的增生指数、成纤维细胞数密度及胶原纤维面积密度。结果青蒿素、青蒿琥酯配制的膏剂组均较对照组瘢痕平坦、柔软且成纤维细胞胞体变小，胶原纤维排列较稀疏、整齐。青蒿素组增生指数、成纤维细胞数密度、胶原纤维面积密度分别为（1．452±0．27）、（3638．245±463．0）细胞／mm^2、（32．29±6．9）％；青蒿琥酯组增生指数、成纤维细胞数密度、胶原纤维面积密度分别为（1．445±0．24）、（3585．016±638．9）细胞／mm^2、（34．74±8．27）％。两实验组检测指标均小于基质对照组及空白对照组，P〈0．01。青蒿素组与青蒿琥酯组相比较，P〉0．05，差异无统计学意义。结论青蒿素、青蒿琥酯膏可有效地抑制动物实验性皮肤瘢痕。
Objective To evaluate the preventive and therapeutic effects of artemisinin and artesunate on hypertrophic scar in rabbit ears. Methods Full-thickness wounds to cartilage were created in New Zealand white rabbit ears to establish animal models of hypertrophic scar. Cream was prepared with artemisinin or artesunate. A total of 96 hypertrophic scars were divided into 4 groups to receive the treatment with artemisinin cream, artesunate cream, cream vehicle （vehicle control） or no treatment （blank control） 28 days after wounds were created. After 28-day treatment, animals were scarified, scars were incised and examined with HE-staining and VG-staining. Hypertrophy index, numerical density of fibroblasts and area density of collagen fibers were calculated. Results Compared with vehicle and blank controls, the scars were softer and flatter, the volume of fibroblasts decreased, and collagen fibers appeared to be more regulated and sparse in artemisinin or artesunate cream-treated groups. The Hypertrophy index, numerical density of fibroblasts, area density of collagen fibers were （1.452 ± 0.27）, （3638.245 ±463.0） cells/mm2, （32.29± 6.9）% in artemisinin cream-treated group, respectively, （1.445 ± 0.24）, （3585.016 ±638.9） cells/mm^2, （34.74±8.27）% in artesunate cream-treated group, respectively. All the three parameters were significantly reduced in artemisinin and artesunate groups than in blank and vehicle control groups （all P 〈 0.01 ）, but no significant difference was found between artemisinin and artesunate groups （P 〉 0.05）. Conclusion Artemisinin and artesunate cream has a reliable efficacy in the prevention and treatment of hypertrophic scar in animal models.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2009年第6期421-424,共4页
Chinese Journal of Dermatology
基金
广西自然科学基金（桂科青0447033）,中国高等教育学会“十一五”教育科学研究规划立项课题（06AIL0770110）,广西壮族自治区教育厅,学位与研究生教育改革和发展专项课题（2007重-16）,新世纪广西高等教育教学改革工程“十一五”第二批立项（一般资助项目48）
关键词
青蒿素
青蒿琥酯
瘢痕
模型
动物
Artemisinin
Artesunate
Cicatrix
Models, animal
