摘要
目的:探讨神经母细胞瘤(NB)发生发展机理。方法:应用原位杂交技术对20例 NB 标本中细胞周期正、负调控基因 p16和 cyclin D_1表达进行检测。结果:本组标本无1例有 p16表达阳性细胞。17例可见 cyclin D_1表达阳性细胞,表达指数4.3%～55.3%。年龄<1岁和神经节神经母细胞瘤标本中 cyclin D_1表示指数低于年龄>1岁 NB,但差异无显著性;Ⅲ～Ⅳ期、预后差型及肿瘤广泛浸润转移标本中 cyclin D_1表达指数显著高于Ⅰ～Ⅱ及ⅣS期、预后好型及肿瘤能切除者。cyclin D_1表达指数与 MKI 呈正相关。与生存时间呈负相关。结论:p16基因表达低,不能抑制 cyclin D_1表达导致的细胞增殖周期加快,在 NB 的发生发展中具有重要作用。cyclin D_1表达高,患儿预后差。检测 cyclin D_1表达,可为判断 NB 恶性程度提供理论依据。
Objective:To assess the expression of p16 and cyclin D_1 genes in neuroblastoma (NB).Methods:Expression of p16 and cyclin D_1 genes were assessed by in-situ hybridization in twenty NB specimens.Results:No expression of p16 gene was detected in the 20 specimens. while expression of cyclin D_1 was found in 17 specimens.The expression index ranged from 4.3% to 55.3%.The expression index was lower in patients under 1 year of age comparing to that of pa- tients over 1 year old although this failed to reach statistical significance.The cyclin D_1 expression index of specimens from stage Ⅲ-Ⅳ patients(with poor prognosis)was significantly higher than that from stage Ⅰ-Ⅱ and ⅣS patients(with fair prognosis).Cyclin D_1 expression index correlates with Mitosis karyorrhexis Index(MKI).There was a negative correlation between cyclin D_1 ex- pression index and the survival.Conclusions:Low expression of p16 may be associated with over- expression of cyclin D_1,resulting in defect of cell-cycle control.Prognosis was poor in those with high cyclin D_1 expression.Cyclin D_1 may be used as a tumor marker.
出处
《中华小儿外科杂志》
CSCD
1998年第3期132-134,193,共3页
Chinese Journal of Pediatric Surgery
