摘要
目的探讨水通道蛋白（AQP）-1与椎间盘终板细胞凋亡的关系及意义。方法将23个含终板的完整椎间盘分为4组：A组直接把取出的新鲜完整的椎间盘作为对照（椎间盘×5），B组为无干预组[进行培养但不加白细胞介素（IL）-1β，椎间盘×6]，C组加入10μ/L IL-1β（椎间盘数×6），D组加入30μg／L IL-1β（椎间盘数×6）。B、C、D组培养2周后取组织的相邻切片，免疫组织化学检测AQP-1和Caspase-3的表达。结果免疫组织化学显示A组中AQP-1和Caspase-3阳性细胞率分别为（88．4±1．1）％和（5．5±0．8）％，B组阳性细胞率分别为（86．7±1．4）％和（6．3±1．6）％，C组中阳性细胞率分别为（42．8±2．2）％和（62．0±1．7）％，D组阳性细胞率分别为（30．5±2．O）％和（71．8±1．23）％，AQP-1与Caspase-3表达呈负相关。结论AQP—1表达减少与终板细胞的凋亡密切相关。
Objective To investigate the relationship between aquaporin-1 and apoptosis of enplate chondrocytes in the intervertebral disc. Methods 23 naturally constrained intervertebral disc/endplate units were divided into 4 groups randomly. Group A （n = 5 ） were used as the control, group B, C, D （n = 6,6,6 ） were cultured in multi-well plate 2 weeks. Group B were not treated by IL-1β, and group C, D were treated by IL-1β 10μ/L and 30μg/L separately. The expression of aquaporin-1 and Caspase-3 were examined by immunohistoehemical staining. Results The immunohistochemical staining showed that the rate of AQP-1 and Caspase-3 positive-staining cells in group A were （ 88.4 ± 1.1 ） % and （ 5.5± 0.8） %. The rate of AQP-1 and Caspase-3 positive-straining cells in group B were （ 86.7 ± 1.4） % and （6.3 ± 1.6）%. The rate of AQP-1 and Caspase-3 positive-staining cells in group C and group D were （42.8 ± 2.2） %, （62.0 ±1.7 ） % and （ 30.5±2.0） %, （71.8 ±1.23 ） %. As the concentrations of IL- 1β increased ,the expression of AQP-1 was significantly decreased and the expression of Caspase-3 was significantly increased. The expression AQP-1 and Caspase-3 was a negative correlation. Conclusion The decreased expression of AQP-1 may lead to apoptosis of enplate chondrocytes in the intervertebral disc, and it may be as a target to prevent apoptosis of enplate chondrocytes, and then treat or delay the intervertebral disc degeneration.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第6期724-725,共2页
Chinese Journal of Experimental Surgery
基金
湖北省自然科学基金资助项目（2005ABA316）
关键词
椎间盘
终板细胞
水通道蛋白-1
凋亡
Interverteral disc
Endplate chondrocytes
AQP-1
Apoptosis
