摘要
以青花菜幼嫩雌蕊及根尖为材料采用常规压片法进行染色体核型分析。结果表明:雌蕊子房部位处于有丝分裂中期的细胞较花柱和柱头部位多;与根尖相比,幼嫩子房中处于分裂中期的细胞较多,且中期染色体在形态上与根尖无明显区别,随体均清晰可见。两种材料获得的核型公式均为2n=2x=18=8m+10sm(2sat),其中第3、4、7、8对为中着丝粒染色体,第1、2、5、6、9对为近中着丝粒染色体,随体均位于第6对染色体上。核型分类均为基本对称型的2A型。用雌蕊为材料进行核型分析,为染色体较小且具随体的十字花科作物的细胞学研究提供了新的方法。
In this article, we studied broccoli karyotype using immature pistil and root tip via squash method. The results indicated that there were more metaphase stage celles in ovary than in stigma and style. Compared with the root tip, the immature ovary possessed more cells which were at metaphase stage; There were no obvious differences in patterns of the morphology chromosome in both materials, we could see satellites in both ceils of two materials ; No matter which material was used, the karyotype formula both were 2n = 2x = 18=8m ＋10sm （2sat）, with 4 metacentric pairs （3, 4, 7, 8） and 5 acrocentric pairs （1, 2, 5, 6, 9） chromosomes, satellites all were observed at the sixth pair of chromosomes. The karyotype all belong to 2A, presented a ultimately symmetry karyotype. In addition, the pistil was more plentiful than root tip when used as preparation of chromosome material. This provided a new method about cytology studies for crucifer crops which have smaller chromosome and satellites.
出处
《园艺学报》
CAS
CSCD
北大核心
2009年第5期727-730,共4页
Acta Horticulturae Sinica
基金
江苏省自然科学基金项目(SBK20082174)
关键词
青花菜
雌蕊
根尖
染色体核型
broccoli
pistil
root tip
chromosome karyotype
