摘要
目的探讨抑癌基因p53突变与肝细胞癌(HCC)预后的关系。方法采用聚合酶链反应-限制性片段长度多态性分析技术,检测HCC p53基因第7外显子点突变,研究55例来自南宁地区的HCC。结果 34例HCC p53基因249密码子发生点突变,伴HCC转移者16例,未转移者18例;Ⅰ级分化1例,Ⅱ～Ⅲ级15例,Ⅳ级18例。p53基因突变型的HCC病人与非突变型的HCC病人1年、3年和5年生存率两两比较有显著性差异(P<0.05)。结论 p53基因突变与HCC的恶性程度及预后有关。
Objective To study the relationship between mutation of suppressor gene p53 and prognosis of hepatocellular carcinoma (HCC). Methods Fifty-five cases of HCC from Naning prefecture were studied by combined use of polymerase chain reaction and restrictive fragment length polymorphism analysis to examine the exon 7 of p53 gene in HCC. Results In 55 cases of HCC,mutation at codon 249 of p53 gene was found in 34 cases with metastassi in 16 cases and without metastasis in 18. The total of p53 gene mutation was 1,15, and 18 cases in stage Ⅰ, stages Ⅱ - Ⅲ , and stage Ⅳ differentiated HCC, respectively. The 1-year, 3-year, and 5-year survival rates of the patients with mutation of p53 gene versus the group of those with no mutation showed statistically significant difference respectively (P<0.05). Conclusion p53 gene mutation is correlated wish the malignant degree and prognosis of HCC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1998年第4期316-317,共2页
Chinese Journal of Experimental Surgery
关键词
肝肿瘤
肝细胞癌
P53基因
基因突变
Hepatocellular carcinoma
p53 gene
Polymerase
chain reaction Prognosis
