摘要
目的:探讨冷应激对雏鸡能量代谢的影响。方法:采用公雏鸡为实验动物,进行急性(0.25,1,3,6,12与24 h)与慢性(5,10与20 d)冷应激处理(12±1℃),检测了雏鸡腓肠肌解偶联蛋白(UCP)mRNA的表达水平,血清葡萄糖(BG),游离脂肪酸(FFA),胰岛素(INS)以及血浆胰高血糖素(GLU)的含量。结果:急性应激时,UCP mRNA的水平随应激时间的延长呈上升趋势,INS与FFA含量随应激时间的延长呈现波动性变化,GLU含量随应激时间的延长呈现上升趋势,BG含量随应激时间的延长呈现先升高后降低的趋势;慢性应激时,UCP mRNA的水平随应激时间的延长呈上升趋势,INS、GLU、BG以及FFA含量随应激时间的延长均呈上升趋势。结论:冷暴露可以使雏鸡的能量代谢发生改变,而且不同程度的冷应激对机体的能量代谢会产生不同的影响。
Aim： To investigate the effect of cold stress on the energy metabolism in Yisha chickens. Methods： Male Yisha chickens were subjected to acute（0.25, 1, 3, 6, 12 and 24 h） and chrunic（5, 10 and 20 d） cold stress（12± 1℃）. This study detected uncoupling protcin（UCP） mRNA levels in gastrocnemius, glucagons（GLU） content in blood plasma and insulin（INS）, blood glucose（BG） and free fatty acid（FFA） content in serum in the chicken. Results： The results were as follow： with the time lapsing during acute cold stress, UCP mRNA levels gradually increased, the content of INS and FFA showed fluctuant change, GLU content gradually increased, and BG content first increased and then decreased. During chronic cold stress, UCP mRNA levels significantly increased compared with their control group at every stress time point, and the content of INS, GLU, BG and FFA were all gradually increased with the time lapsing. Conclusion： Cold stress could change the energy metabolism in chickens. And the different extent cold stress would produce different effects on the energy metabolism.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2009年第2期172-176,共5页
Chinese Journal of Applied Physiology
