摘要
参考海葵Nematostella vectensis在GenBank中的β-actin基因mRNA部分序列(XM_001630533)设计引物,对仿刺参Apostichopus japonicus进行特异的PCR扩增,将扩增产物测序分析,再根据测序结果设计仿刺参专用β-actin引物ACT1/ACT2,对不同退火温度和循环次数条件下的该基因RT—PCR产物进行了对比。本研究中首次克隆了仿刺参的β-actin基因,为今后仿刺参目的基因表达的半定量分析研究奠定了基础。
The β-actin primer ACT1/ACT2 of sea cucumber Apostichopus japonicus was designed according to the β-actin mRNA of sea anemone Nematostella vectensis from GenBank（ XM_001630533 ）. RT- PCR products amplified and sequenced by this pair of primer were studied under different conditions of annealing temperature and amplified cycles. It was the first β- actin gene cloned in the sea cucumber which showed that PCR product amplified by this pair of primer can be used as endogenous control for gene semi - quantitative research.
出处
《大连水产学院学报》
CSCD
北大核心
2009年第2期176-180,共5页
Journal of Dalian Fisheries University
基金
国家自然科学基金资助项目(30371099)
辽宁省自然科学基金资助项目(20052139)
关键词
仿刺参
Β-ACTIN
分子内标
Apostichopus japonicus
β- actin
endogenous molecular marker
