摘要
目的用氯胺T法标记眼镜蛇神经毒素(Neurotoxin,NTX),观察其在大鼠体内的分布。方法实验组和对照组的大鼠分别单次静脉注射125I-NTX50μg.kg-1和相同剂量的Na125I与NTX的临时混合液,于给药后30min和2h分两批处死,取各脏器组织样本称重并计算每g组织的cpm。用组织与血液的脉冲比值作为放射性相对掺入量的标准,用实验组(e)和对照组(c)相对放射性掺入量的比值(e/c)作为判断125I-NTX在大鼠各脏器组织分布多少的依据。结果大鼠静脉给125I-NTX50μg.kg-1后30min,分布最多的是肾脏,每g组织放射性达44cpm,为对照组的11倍。膀胱及尿、肺、肠及内容物、肾上腺亦有较高分布。给药后2h,分布最多的仍是肾脏,但比0.5h时减少一半左右。结论NTX主要分布在肾脏,肾脏以外的实质性脏器分布均较少,脑中分布最低。
OBJECTIVE To study the distribution of Neurotoxin from Naja naja atra in rat, the NTX was labeled with ^125I using the method of Chloramine-T. METHODS The experimental group and the control group were received single intravenous administration of ^125I-NTX （50μg·kg^-1） and the same dose of temporary mixed liquor of Na^125I and NTX. The rats are executed in batch after administration in 0.5h and 2h, and then every tissue was obtained. The distribution of NTX was measured by relative incorporation of radioactivity, which was obtained by dividing the cpm per g wet weight of the various tissues by the cpm per 0.2mL of blood from the corresponding animal. The quantity of NTX in tissues was measured by the ratio of relative incorporation radioactivity in experimental group and control group. RESULTS After 0.5h of administration, the kidney contains the most of ^125I-NTX and the relative radioactivity reached 44 cpm per g wet weight of tissue, which was 11 fold higher than the control group. Bladder with urine, small intestine and adrenal also contained more NTX. After 2 h administration, the kidney always contained the most of ^125I-NTX but the quantity decrease to one half of the 0.5h of administration.CONCLUSIONS It shows that, NTX mainly distributes in kidney, besides this tissue, there is no more distribution in parenchyma tissues, especially the brain.
出处
《海峡药学》
2009年第5期36-38,共3页
Strait Pharmaceutical Journal
