摘要
目的:评价不同产地的黄连药材质量。方法:采用HPLC法测定不同产地黄连中盐酸小檗碱的含量。色谱条件:diamonsil C18色谱柱(4.6mm×250nm,5μm),流动相为乙睛-0.1%磷酸溶液(50:50),检测波长265nm。结果:盐酸小檗碱在0.25～4.0μg范围内线形关系良好(r=0.9999),平均回收率为98.7%,RSD=0.34%,8个样品黄连中盐酸小檗碱的含量为5.8%～8.6%。结论:不同产地黄连在盐酸小檗碱含量上有一定差异,但均符合现行《中国药典》2005版规定,总体质量良好。
Objective To evaluate the qualities of Rhizoma Coptidis from different areas. MethodsThe content of berberine hydrochloride in Rhizoma Coptidis was determined with HPLC. The HPLC was performed on a diamonsil C18 （4.6 mm×250 nm,5μm）column, the mobile phase was acetonitrile -0. 1% phosphoric acid solution（50： 50）, and the detection wavelength was 265 nm. Results The berberine hydroehloride showed good linearity over the range of 0.25-4.0μg（ r = 0. 9999）, the average recovery was 98.7% , RSD = 0.34%, the contents of berberine hydroehloride were 5.8%-8.6% in 8 samples. Conelusion The contents of berberine hydroehloride in Rhizoma Coptidis have difference from different areas, and all meet the requirements in Chinese Pharmacopoeia.
出处
《郧阳医学院学报》
2009年第2期151-152,155,共3页
Journal of Yunyang Medical College
关键词
黄连
HPLC
盐酸小檗碱
Rhizoma Coptidis
HPLC
Berberine hydrochloride
