摘要
为了探讨小麦中wasi基因的结构与功能,以大麦basi基因的EST为基础,通过电子克隆结合RT-PCR方法分离克隆获得了608 bp的小麦α-淀粉酶/枯草杆菌蛋白酶抑制剂基因TaWASI2。序列分析显示,所克隆的基因片段包含513 bp的开放阅读框,编码一个含有170个氨基酸的多肽,具有保守的STI结构域。通过半定量PCR对TaWASI2基因的表达特性分析表明:TaWASI2基因在种子发育过程中表达量逐渐增加;在胚乳中表达高于其他组织。
In order to provide an insight into molecular characteristics and putative epigenetic fuctions of ASI in wheat,a gene with complete open reading frame （ORF） designated as TaWASI2 was identified in wheat by in silico cloning approach and cloned by RT-PCR. Analysis of the sequence of the gene showed that he cDNA sequence was 608 bp,including an ORF of 513 bp,which encoded a peptide of 170 amino acids. Analysis of the deduced amino acid sequence of the gene showed that TaWASI2 contained a STI conserved domains. Expression patterns of TaWASI2 were studied via SQ-RT-PCR and the results showed that the expression level of TaWASI2 was gradually increased with the developing grains, and it reached the highest level at the time point of 20 days after post floral it expressed at high level in endosperm than other organs.
出处
《西北农业学报》
CAS
CSCD
北大核心
2009年第3期74-77,共4页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金(30370877)
河南省杰出青年基金(0512001600)
